Objective:To investigate the effects of Daidzein on behavior of chronic stress depression rats and the expression of hippocampal brain-derived neurotrophic factor ( BDNF ) , neuropeptide Y ( NPY ) and non-specific immune regulation.Methods: 40 healthy adult male SD rats with body weight(210±19)g,clean grade,were chosen and fed with 1%sucrose solution for 4 d to change drinking habits.On the fifth day rats were subjected to water deprivation for 24 h without fasting.On the sixth day rats were fed with 1%surcrose solution.4 h later, preference of 1% surcrose solution was examined.According to the 1% sucrose solution preference and weight rats were randomly divided into 4 groups,normal control group(CG),model control group,(MG),fluoxetine group(FG,10.0 mg/kg),daidzein group(DG,80.0 mg/kg).At the same time of establishing model,rats were administered orally once a day for 32 d.The depression model was established by chronic unpredictable mild stress model and separation.The behavioral changes of the rats were observed, and expression of BNDF in hippocampus and NPY was measured by Western blot technology and immunohistochemistry.It was observed the proliferation function of lymphocytes,spleen index,the number of peripheral blood leukocytes and antibody-secreting cell function.Results: Compared with the normal control group(CG),the weight of rats with chronic stress protocol was lower, 1%sucrose consumption decreased,scores of rats in the open field test dropped significantly,the immobility time in the forced swimming test prolonged,the level of expression of BNDF and NPY decreased,all the differences above were statistically sig-nificant(P<0.01).Compared with model group,weight of rats in fluoxetine treatment group(FG) and daidzein treatment group(DG)in-creased,sugar consumption,scores in the open field test and the levels of expression of BNDF and NPY significantly increased,the differences were statistically significant( P<0.05 or P<0.01 ).The number of peripheral blood leukocytes and antibody-secreting cell function and proliferation of lymphocytes force in daidzein treatment group was significantly higher than the model group,daidzein dose spleen index was significantly higher than the model group(P<0.01).Conclusion: The daidzein can antagonize depressive symptoms in chronic stress mice,daidzein may increased content of BDNF in hippocampus and NPY protein, and enhanced the role of humoral immune response and lymphocyte proliferation in rats with chronic stress model.The mechanisms of antidepressant effects of daidzein might be related to the increase of content of BDNF in hippocampus and NPY protein and non -specific immune regulation.%目的：探讨大豆甙元（ Daidzein）对慢性应激抑郁模型大鼠海马脑源性神经生长因子（ BDNF）、神经肽Y （NPY）蛋白表达和非特异免疫调节作用的影响。方法：取健康成年SD大鼠40只，雄性，体质量（210±19）g，清洁级，用1％蔗糖水喂养4 d，使动物原来的饮水习惯发生改变。第5天开始24 h禁水，但不禁食，第6天给予1％蔗糖水4 h，测1％蔗糖水的偏嗜度。根据1％蔗糖水偏嗜度和体重随机分成4组，每组10只，糖水偏嗜度和体重各组间没有显著差异（ P＞0.05）。正常对照组（ CG）、模型对照组（ MG）、氟西汀对照组（ FG，10.0 mg／kg）、大豆甙元组（ DG，80.0 mg／kg）。造模同时灌胃给药，每日1次，连续给药32 d。造模方法为慢性轻度不可预见性的应激模型加孤养。观察大鼠的行为改变，免疫蛋白印记（ Western blot）技术和免疫组织化学法检测海马BNDF和NPY表达，观察淋巴细胞增值功能，脾脏指数，外周血白细胞数目及抗体分泌细胞功能。结果：与正常对照组相比慢性应急刺激大鼠体重、蔗糖消耗量及大鼠旷场试验得分显著降低，游泳不动时间延长，海马BNDF和NPY表达水平低下，差异有统计学意义（P＜0.01）。氟西汀治疗组和大豆甙元治疗组与模型对照组相比大鼠体重、蔗糖消耗量、大鼠旷场试验得分以及海马BNDF和NPY表达水平显著增加，差异有统计学意义（ P＜0.05或P＜0.01）。大豆甙元组大鼠外周血白细胞数目、抗体分泌细胞功能及淋巴细胞增殖力显著高于模型组。大豆甙元剂量脾脏指数显著高于模型组（ P＜0.01）。结论：大豆甙元能显著拮抗慢性应激模型大鼠抑郁症状，大豆甙元可增加海马内BDNF和NPY蛋白的含量，并且有增强慢性应激模型大鼠体液免疫应答和淋巴细胞增殖能力的作用，大豆甙元拮抗慢性应激模型大鼠抑郁作用的机制可能与大豆甙元治疗后海马中BDNF和NPY蛋白含量增加及免疫调节作用有关。
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