连翘根提取物（FSEER）对化疗后小鼠骨髓和免疫抑制的影响

摘要

Objective:To observe the effect of FSEER on the immunosuppressive and bone-marrow-suppressive mice after chemotherapy,and explore the mechanism of hematopoietic and immunologic function in mice accentuated by FSEER.Methods: Mice were injected cyclophosphamide(Cy)except control group,then randomly divided into mode group(saline),FSEER groups[120,60 mg/( kg· d) ].The spleen index( SI) of all mice was calculated respectively.Flow cytometry instrument testing mice peripheral blood lymphocytes CD3,CD4,CD8 change.The content of TNF-α,IL-2 and IL-12 in mice serum were measured by ELISA kits.Morphological images of bone marrow of the mice were observed under the microscope after Wright-Giemsa′s staining.Results:The spleen index( SI) was increased in both of the two FSEER groups.ELISA analyses showed that the content of TNF-αand IL-2 was increased in both of the two FSEER groups.The population of CD3+CD4+T lymphocyte and the ratio of CD4+/CD8+were all increased in the low dose experimental group.After treated with FSEER, the hematopoietic depression was improved significantly.Conclusion: FSEER can improve the state of immunosuppressive and myelosuppressive mice caused by Cy thus could alleviate the side effect of chemotherapy ef-fectively.%目的：观察连翘根提取物（ FSEER）对环磷酰胺（ Cy）所致小鼠骨髓和免疫抑制作用的影响，探讨FSEER促进化疗后小鼠造血功能和免疫功能的作用及机制。方法：将小鼠随机分为4组：正常对照组，模型组，FSEER高、低剂量组。除正常对照组外，其余各组小鼠均一次性腹腔注射Cy（200 mg／kg）。第2天开始，对FSEER高、低剂量组小鼠灌胃不同剂量的FSEER[120、60 mg／（kg· d）]，模型对照组小鼠给予等量的生理盐水，连续给予10 d。分离各组小鼠脾脏，分别计算脾脏指数（ SI）。流式细胞仪检测小鼠外周血淋巴细胞CD3、CD4、CD8的变化。用ELISA试剂盒检测小鼠血清中TNF-α、IL-2、IL-12的含量，股骨骨髓涂片经瑞式-吉姆萨染色后观察骨髓细胞形态变化。结果：不同剂量的FSEER组小鼠的脾脏指数增加，外周血中TNF-α和IL-2的含量增高。一定剂量的FSEER可以提高外周血CD3＋CD4＋T淋巴细胞比例和CD4＋／CD8＋的比值，改善骨髓的抑制状态。结论：连翘根醇提物（ FSEER）可以显著提高小鼠机体的免疫功能，恢复由Cy诱导的小鼠免疫抑制状态。