不同磷脂肽段诱导的实验性自身免疫性脑脊髓炎模型病理特征多样性的研究

摘要

目的：探索是否不同磷脂肽段可以诱导Lewis大鼠产生不同的病理学表现。方法：采用髓鞘碱性蛋白82－99（ MBP82－99）、MBP68－86、髓鞘少突胶质细胞糖蛋白35－55（ MOG35－55）免疫Lewis大鼠，每天进行神经功能评分。免疫后取各组大鼠大脑、小脑、脑干和脊髓组织，观察炎性细胞浸润部位和浸润程度、有无脱髓鞘和轴索损害。结果：MBP68－86和MBP82－99两组大鼠的大脑、脑干、小脑和脊髓组织均有炎性细胞浸润，脊髓炎症程度重于其他部位；MBP68－86和MBP82－99诱导的脊髓炎症程度无统计学差异；MOG35－55组大鼠仅脊髓组织受累，且炎症程度明显低于MBP组，其余各组未见炎性细胞浸润。各组大鼠神经组织均未见脱髓鞘和轴索受累。结论：不同磷脂肽段诱导Lewis大鼠神经组织炎性细胞浸润的分布和程度不同。%Objective:To explore whether different phospholipid peptides could induce different pathological features of EAE in Lewis rats.Methods:Lewis rats were immunized with myelin basic protein 82-99 (MBP82-99),MBP68-86,and myelin oligodendroglia glycoprotein 35-55 (MOG35-55),respectively,and evaluated everyday for neurological scores.The cerebrum,brain stem,cerebellum and spinal cord of every rat were removed and pathological features observed.Results: Rats immunized with the two MBP peptides exhibited neurological signs of EAE wherein the central nervous system had extensive inflammation infiltration.The number of infiltrates in spinal cords in the two MBP peptides groups was higher than that in the cerebrums,brain stems and cerebellums.In spinal cords, there was no statistical difference of infiltrates between MBP82-99 and MBP68-86 groups;however,the both groups had more infiltrates than MOG35-55 group.Inflammatory cells were observed only in spinal cords of animals immunized with MOG35-55.Conclusion:This study provides certain evidence for understanding the diversity of pathological manifestations of EAE in Lewis rats.