IL-10基因多态性与溃疡性结肠炎易感性的关系及对临床预后的影响

摘要

目的：探讨IL-10多态位点的基因多态性与溃疡性结肠炎的易感性及对临床预后的影响。方法：采用病例-对照研究设计，选取80例溃疡性结肠炎患者作为病例组，另外选性别和年龄匹配的健康受试者作为对照组。所有患者治疗前抽取空腹静脉血并提取DNA，设计-819 T／C（rs1800871）、-592 A／C（rs1800872）、-1082 G／A（rs1800896）PCR引物进行PCR扩增，扩增产物酶切后进行琼脂糖凝胶电泳以确定基因类型，采用Logistic回归计算校正相对危险度（ OR）和95％置信区间（95％CI）评价基因多态性与溃疡性结肠炎的易感性，并分析对临床预后的影响。结果：（1）病例组患者IL-10多态位点rs1800896基因类型AA、GG和AG分布频率与对照组受试者差异具有统计学意义（ P＜0.01）；（2）与rs1800896基因型AA比较，基因型为GG的患者溃疡性结肠炎危险性显著升高（P＜0.01），并且临床缓解率显著降低（P＜0.01）；（3）病例组患者IL-10多态位点rs1800871基因类型CC、CT和TT分布频率与对照组受试者差异无统计学意义（P＞0.05）；（4）病例组患者IL-10多态位点rs1800872基因类型AA、AC和CC分布频率与对照组受试者差异无统计学意义（ P＞0.05）。结论：IL-10多态位点rs1800896基因类型GG可增加溃疡性结肠炎的易感性，并且显著降低患者的临床预后。%Objective:To study the correllation of genetic polymorphisms of IL-10 polymorphic loci with ulcerative colitis sus-ceptibility and its influence on clinical outcomes.Methods:A total of 80 patients with ulcerative colitis were selected as case group and the others by sex and age matched healthy subjects as control group according to the case-control study design.Peripheral venous blood samples were drawn and DNA was extracted from all subjects prior to treatment.PCR primers of -819T/C(rs1800871),-592A/C (rs1800872),-1082G/A(rs1800896) were designed for PCR amplification.The fragments produced from human genomic DNA were performed by restriction enzyme digestion of amplified PCR products,and further separated using agarose gel electrophoresis.Relative risk(OR) and 95%confidence interval(95%CI) were calculated by the Logistic regression,in order to evaluate the correlation of IL-10 gene polymorphism with susceptibility of ulcerative colitis and clinical outcomes.Results:(1)The distribution frequency of genotype AA,GG and AG of polymorphic loci rs1800896 in cases patients were significantly different from that in control group(P<0.01).(2) Compared to rs1800896 genotype AA,genotype GG were significantly associated with increased risk for ulcerative colitis(P<0.01)and the decreased clinical remission rate ( P<0.01 ).( 3 ) The distribution frequency of genotype CC, CT and TT of polymorphic loci rs1800871 were not significantly different between groups(P>0.05).(4)The distribution frequency of genotype AA,AC and CC of polymorphic loci rs1800872 were not significantly different between groups(P>0.05).Conclusion:IL-10 polymorphic loci rs1800896 genotype GG would be associated with increased susceptibility to ulcerative colitis,and poor prognosis of patients.