血清IL-34水平与SLE疾病活动度关系的研究

摘要

Objective:To investigate levels of serum IL-34 in SLE patients,and correlation between concentrations of serum IL-34 and other established serum markers and disease activity indexes.Methods: In all,78 SLE patients and 53 healthy controls were enrolled in the research.Enzyme-linked immunosorbent assay(ELISA) was employed to measuring the concentrations of serological IL-34.Then,serum IL-34 levels between SLE group and healthy controls were analyzed by Mann-Whitney U test.Meanwhile,the correlation between the serum IL-34 levels and disease activity indexes and other established serum markers were assessed.Serum IL-34 levels were significantly higher in SLE patients compared to healthy controls[(Median,128.9 pg/ml) vs (Median,52.4 pg/ml),P<0.001].Results: Their levels were remarkably associated with accumulation of the clinical features of SLE.Additionally,IL-34 titers were positively correlated with the SLE disease activity indexes,anti-double stranded DNA antibody(anti-dsDNA)titers and C-reactive protein(CRP)levels,but inversely with C3 levels.Conclusion: Serum IL-34 could be a candidate biomarker for SLE as the elevated serum levels in treatment-naive SLE patients and its association with SLE disease activity.%目的:检测系统性红斑狼疮(Systemic lupus erythematosus,SLE)患者血清白细胞介素(Interleukin,IL)-34水平,并进一步探讨IL-34与临床和实验室指标及SLE疾病活动度的关系.方法:采用酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)测定78例SLE组及53名正常对照组血清IL-34水平,SLE组与正常组血清IL-34水平分析采用曼-惠特尼U检验(Mann-Whitney U test).结果:与正常对照相比,SLE组血清IL-34水平明显高于正常对照组[(Median,128.9 pg/ml) vs (Median,52.4 pg/ml),P<0.001],差异有统计学意义.IL-34水平与SLE疾病活动度、双链DNA抗体(anti-double stranded DNA antibody,anti-dsDNA)滴度、C反应蛋白(C-reactive protein,CRP)呈正相关,与C3水平呈负相关.结论:血清IL-34可能成为SLE的一血清标志物,因其在SLE患者中升高,且与疾病活动度相关.