Rab7基因沉默对巨噬细胞中R848激活的细胞因子及MAPK信号通路影响

摘要

目的:本实验阐述了Rab7对Raw264.7巨噬细胞中R848激活TLR7(Toll like receptor-7)所产生的细胞因子的影响,并探讨Rab7对MAPK信号转导的影响.方法:使用Rab7刺激silence-Rab7-Raw264.7细胞,检测R848激活TLR7后产生的TNF-α、IL-6、IFN-α、IFN-β与IP-10,通过Western blot检测MAPK的磷酸化水平,分析Rab7对MAPK的信号转导的作用.结果:Rab7对TLR7激活后细胞因子的产生具有抑制作用,Rab7对MAPK信号通路具有抑制作用.结论:本实验结果进一步说明Rab7 是TLR7信号转导通路的负向调控因子,并且参与到MAPK信号通路中.%Objective:To investigate the effect of Rab7 on cytokine induced by TLR7 (Toll like receptor-7) R848 activated in Raw264.7,and discusses the influence of Rab7 on MAPK signal transduction.Methods: TLR7 downstream cytokines such as TNF-α,IL-6,IFN-α,IFN-β and IP-10 activated by R848 were detected through Q-PCR in Rab7 silenced mouse macrophages,and then analysis of phosphorylation of MAPK determined with Western blot showed the effect of Rab7 on signal transduction of MAPK.Results: Rab7 inhibit production of cytokine activated by TLR7,and also,Rab7 had an inhibitory effect on MAPK signal pathway.Conclusion: The experimental results further illustrate that the Rab7 is the TLR7 signal transduction pathway negative regulatory factor,and to participate in MAPK signaling pathway.