精子DN A碎片指数与精液参数的相关性研究

摘要

目的：探讨男性精子DNA碎片与精液常规参数及精子顶体酶活性之间的关系。方法：收集生殖中心门诊553例男性患者精液标本，采用改良精子染色质扩散实验（SCD）检测精子 DNA 碎片指数（DFI），按精子DFI结果分为DFI＜15％（I组）、15％≤DFI＜25％（II组）、DFI≥25％（III组），同时检测精子密度、总数、（a＋b）级精子百分率、畸形率及顶体酶活性，对DFI与精液各项参数的相关性进行分析。结果：精子密度及精子总数与DFI无显著相关性；前向运动精子百分率随DFI升高逐渐降低，III组与I组间存在显著性差异；精子正常形态率随DFI的增加有下降的趋势，但是差异不显著；精子顶体酶活性随DFI升高而降低，组间均有显著性差异。结论：精子DNA碎片指数与前向精子百分率及精子顶体酶活性呈负相关。因此DFI可作为评估精子功能的一个较好的参考指标。%Objectives:To explore the relationship between the male sperm DNA fragmentation,semen pa-rameters and acrosome activity.Method:Semen samples of 553 male patients were collected from our center. Sperm DNA fragmentation index(DFI)was detected by Sperm Chromatin Dispersion(SCD)methods.According to the results of sperm DFI,all the values were divided into group I (DFI<15%),group II (15%≤DFI <25%) and group III (DFI≥25%),and the sperm density,count,percentage of progressive motility sperm,morphology and acrosome activity were detected at the same time.The correction of DFI and semen parameters were analyzed. Results:Sperm density and total sperm count had no significant correlation with the DFI.The percentage of progres-sive motility sperm gradually decreased with the augment of DFI,and there were significant differences between group I and group III.Normal sperm morphology rate also decreased with the augment of DFI,and the difference was not significant.Acrosome activity gradually decreased with the augment of DFI as well,with significant differ-ences among the groups.Conclusion:Negative correlations were obtained between both progressive motility sperm and acrosome activity.As a result,DFI is a better reference index for sperm function.