基层医院梅毒螺旋体抗体筛查方法比较及筛查流程探讨

摘要

目的：探讨基层医院梅毒螺旋检测的筛查方法及流程。方法：对9169份标本分别应用酶联免疫方法，胶乳凝集方法，血浆反应素卡试验进行梅毒螺旋体特异性抗体和非特异性抗体的检测，其结果与梅毒螺旋体抗体颗粒凝集试验确证方法的结果相比较。结果：9169份标本中，ELISA 方法检测出的83例阳性标本经 TPPA 确证后79例阳性占95．18％；胶乳快速法检测出的96例阳性标本经 TPPA 确证76例阳性占79．17％；两种方法双阳性的77例标本经 TPPA 确证76例为阳性占98．70％。RPR 法检测到37份阳性标本，经 TPPA 确证34份阳性占91．89％。初筛 ELISA，胶乳快速法，RPR 三种方法均阳性的34例，与经 TPPA 确证均为阳性为100％。结论：对于基层医院可以用 ELISA 方法进行筛查，用胶乳法复检，并用 RPR 法做传染性的检测，适当改进筛查流程确保梅毒螺旋体的检查。%Objectives:To evaluate and investigate the methods for the detection of antibody against trepo-nema pallidum in primary hospitals.Methods:A variety of methods including ELISA,latex agglutination method and rapid plasma regain card test were used to detect the syphilis specific antibody and non -specific antibody in 9169 specimens.The results of all tests were compared with that of treponema pallidum particle agglutination test. Results:Agreements of the TPPA with the ELISA,latex rapid method and rapid plasma regain card test were 95. 18%,79.17% and 91.89 %,respectively.For ELISA and latex rapid method double positive specimens,the a-greement was 98.70%.For the 34 positive specimens in all ELISA,latex rapid method and RPR,the agreement of TPPA was 100 %.Conclusions:Laboratory of primary hospitals can use ELISA method for screening,and emul-sion method for reinspection while RPR method can be used for infectivity detection,which is reliable and effective.