骨形成蛋白(BMP)-2/4，-5与IA型BMP受体在口腔正常上皮、良性病变及癌变中的表达分析

摘要

The aim of the present study was investigate the relationshipsbetween bone morphogenetic proteins (BMPs), BMP receptor type IA and carcinogenesis of oral epithelia. Expressions of BMP-2/4，-5 and BMPR-IA were analyzed in the specimens obtained from buccal mucosa, including 9 cases of normal mucosa (NB), 8 cases of chronic inflammation (NCI), 7 cases of hyperkeratosis (HK), 5 cases of squamous cell papilloma (SCP), 29 cases of squamous cell carcinoma (SCC), 10 cases of epithelium adjacent to carcinoma (EAC) and 6 cases of normal mucosa from hard palate (NHP) by using immunohistochemistry. Results demonstrated that the immunostaining of BMP-2/4，BMP-5 and BMPR-IA was weak and not consistent in normal and benign groups. Except for 3 cases of SCC, all cases of SCC showed positive staining for BMP-2/4, BMP-5 and BMPR-IA. The staining intensity and proportion of the positively stained cells in SCC were markedly increased than in normal and benign groups except for EAC. The metastatic carcinoma cells in lymph nodes were strongly and positively stained for BMP-2/4，BMP-5 and BMPR-IA might be involved in the carcinogenesis of oral epithelia.%认识BMP及其受体与口腔正常上皮及其癌变的关系，有助于深入了解口腔上皮癌变的机理。本文用免疫组织化学方法对BMP-2/4，-5与BMP受体BMPR-IA在口腔颊部粘膜正常上皮、良性病变和癌变中的表达进行观察和半定量分析。标本包括：9例正常上皮(normalbuccal mucosa, NB)、8例慢性炎症(nonspecific chronic inflammation,NCI)、7例过度角化(hyperkeratosis, HK)、5例乳头状瘤(squamous cell papilloma,SCP)、29例鳞癌(squamous cell carcinoma,SCC)、10例癌旁上皮(epithelium immediately adjacent to carcinoma,EAC)以及6例硬腭粘膜上皮(normal mucosa of hard palate,NHP)。结果显示：BMP-2/4，-5与BMPR-IA在口腔粘膜的正常与良性病变上皮中有弱的和不均一的阳性表达，NB与NHP无明显差别。而除3例SCC外，其它SCC几乎均有程度不一的阳性表达。在EAC中的表达接近于SCC，二者明显高于正常与良性组。此外，转移在淋巴结中的癌细胞的BMP-2/4与BMP-5阳性程度略高于原发灶的癌细胞。本文认为：BMP-2/4，-5与BMPR-IA可能参与调控口腔上皮的癌变。