脱细胞羊膜与小肠黏膜下层促进大鼠皮肤缺损修复和血管形成

摘要

Objective To observe the repairing effects and vascularization of the small intestinal submucosa and the acellular amnion for full-thickness skin defects in rats. Methods Two full-thickness skin defects, which were close to the vertebral column, were created on both sides of the dorsum of the SD rats.The skin defects were randomly divided into three groups covered by the acellular amniotic membrane in Group A, the small intestinal submucosa ( SIS) in Group B, and the physiological saline gauze in Group C (the control group) respectively. After two weeks, the sample were harvested for histological evaluation,immunohistochemical staining of K19 and VEGF, as well as for determination of expression levels of VEGF mRNA with RT-PCR. Results Immunohistochemical staining of K19 and VEGF showed that there were more positive cells in group A and B than in group C, and maximum in group A; RT-PCR results showed that the expression levels of VEGF mRNA were higher in group A and B than group C, and maximum in group A too. The results of the statistical analysis of the research data showed that there were statistically significant differences between the three groups (P＜ 0. 05). Conclusion The results indicate that HAM and SIS increases K19 positive cells and enhance expression levels of VEGF, thus promoting skin repair and vascularization in rats.%目的 观察脱细胞羊膜(HAM)与小肠黏膜下层(SIS)促进大鼠皮肤缺损修复和血管形成的作用.方法 SD大鼠24只,在两侧背部各做1个直径为1.8cm圆形全层皮肤缺损.创面随机分为A组、B组和C组.A组HAM覆盖,B组SIS覆盖,C组纱布覆盖.在2周时处死动物取材,HE染色观察皮肤缺损修复情况.免疫组织化学染色检测K19和VEGF,RT-PCR检测VEGF mRNA的表达.结果 A组、B组愈合较好.C组愈合较差.免疫组织化学染色显示,A、B组K19、VEGF阳性细胞显著多于C组,其中A组最多;RT-PCR结果显示,A、B组比C组表达更多的VEGF mRNA其中A组最多,差异显著,有统计学意义(P<0.05).结论 HAM和SIS均能增加皮肤创面组织中K19阳性细胞数,上调VEGF mRNA的表达、增加VEGF的分泌,其中HAM具有更强的促进皮肤缺损修复和血管形成作用.