胃癌组织中幽门螺杆菌L型感染及骨桥蛋白检测的意义

摘要

Objective To detect the helicobacter pylori L-form ( Hp-L) infection and the expression of Osteopontin (OPN) in gastric carcinoma tissues, and to discuss the probable machanism between Hp-L infection and the progression of gastric carcinoma. Methods Gram's staining and immunohistochemical staining ( SP method) were used to detect Hp-L in 120 gastric carcinoma cases. Immunohistochemical staining was also used to evaluate the expression of OPN. Results Hp-L was found in 88 and 85 cases of 120 gastric carcinoma tissues by Gram's and immunohistochemical staining, respectively. 82 (68. 3％) cases were HpL positive by both Gram's and immunohistochemical staining. In the Hp-L positive group, 58 (58/82,70. 73％)cases were OPN positive,while in the Hp-L negative group, 16 cases were OPN positive, which had relationships to the size, invasion and metastasis of the tumor. Conclusion The infection of Hp-L plays an important role in the progression of gastric carcinoma. The increase in the intracellular levels of OPN may be one of the mechanisms .%目的 通过检测胃癌组织中幽门螺杆菌L(HP-L)型感染以及OPN的表达情况,探讨Hp-L型感染对胃癌侵袭、转移生物学行为的影响及可能机制.方法 ①应用革兰染色及免疫组化(SP)法检测120例胃癌组织中的Hp-L型感染情况;②应用免疫组化(SP)法检测胃癌组织中的OPN的表达.分析Hp-L型感染与OPN表达的关系以及和临床病理因素之间的关系.结果 ①120例胃癌组织中有88例革兰染色检出Hp-L型,其阳性率为73.3%(88/120);免疫组化Hp-L型抗原表达阳性率为70.8%(85/120),革兰染色Hp-L型检出和Hp-L型抗原表达同时阳性的病例有82例(两者同时阳性者为Hp-L型阳性),其阳性率为68.3%(82/120).②胃癌组织中Hp-L型阳性组的OPN表达阳性率分别为70.73%(58/82),高于Hp-L型阴性组表达率,且有统计学意义(P＜0.005).经四格表资料χ2检验,胃癌组中Hp-L型阳性与OPN阳性表达呈正相关(r=0.27, P＜0.001).③胃癌中Hp-L型阳性与肿瘤的大小、癌细胞的血管侵袭、浸润深度及淋巴结转移具有相关性(P＜0.001-P＜0.05).结论 Hp-L型感染是引起胃癌侵袭、转移的重要因素之一,促进胃癌的发展,其机制可能与胃癌细胞的OPN表达量增加有关.