p120ctn、Kaiso及matrilysin在非小细胞肺癌中的表达及其意义

摘要

Objective To investigate the expression of pl20ctn, Kaiso and matrilysin proteins in non-small cell lung cancer (NSCLC) tissues, and to analyze their effect on tumorigenesis and development. Methods The expression of p120ctn, Kaiso and matrilysin proteins were detected using immunohistochem-istry in 16 cases of bronchiectasis and 98 cases of NSCLC, including 52 cases of lung squamous cell carcinoma and 46 cases of lung adenocarcinoma. The relationship between pl20ctn, Kaiso, matrilysin and clini-copathologic characteristics was analyzed. Results The abnormal expression rates of p120ctn were 74. 5%, 6. 3% in NSCLC and bronchiectasis specimens respectively, and the difference showed statistical significance (P<0. 001). The ectopic expression rates of Kaiso in lung cancer cells, goblet cells, ciliated cells and basal cells were 63. 3%, 6.3%, 6.3%, 37.5%, respectively, and the difference of ectopic expression rates between lung cancer cells and goblet cells or ciliated cells was statistically significant( P<0. 001). The abnormal expression of p120ctn and cytoplasmic matrilysin expression in patients with NSCLC were both related to histological type (P<0. 05). The ectopic expression of Kaiso in patients with NSCLC was related to the grade of differentiation (P<0.05). Nuclear Kaiso expression was correlated with cytoplasmic matrilysin expression (r=-0. 23, P = 0. 02). Conclusion P120 may play a role in the NSCLC development through co-localization with Kaiso in the cytoplasm, relieving the repression of Kaiso to its target gene, and promoting matrilysin expression.%目的 探讨p120 catenin(p120ctn)、Kaiso和matrilysin在非小细胞肺癌(non-small cell lung cancer,NSCLC)发生发展中的作用.方法 采用免疫组织化学法检测98例NSCLC(52例肺鳞状细胞癌和46例肺腺癌)以及16例支气管扩张症组织中p120ctn、Kaiso和matrilysin的表达情况,分析NSCLC中p120ctn、Kaiso和matrilysin与临床病理特征的关系及三者表达的相关性.结果 在NSCLC组织和支气管扩张症组织中,p120ctn蛋白异常表达率分别为74.5％、6.3％,两组之间表达率比较有统计学差异(P＜0.001);在肺癌细胞、杯状细胞、纤毛细胞、基底细胞中Kaiso蛋白异位表达率分别为63.3％、6.3％、6.3％、37.5％,肺癌细胞与杯状细胞、纤毛细胞表达率比较有统计学差异(P＜0.001).p120ctn蛋白异常表达、matrilysin蛋白细胞质表达均与NSCLC组织学类型密切相关(P＜0.05),Kaiso蛋白异位表达与NSCLC分化程度密切相关(P＜0.05).在NSCLC组织中,Kaiso细胞核表达与matrilysin细胞质表达存在显著负相关(r=-0.23,P=0.02).结论 p120ctn可能通过与Kaiso在胞质内异位表达,解除了Kaiso对靶基因的抑制作用,促进matrilysin的细胞质表达,而参与肺癌的发生发展.