脂质对大鼠肝星状细胞低密度脂蛋白、高密度脂蛋白受体的影响

摘要

Objective To study the effects of lipid (triglyceride and very low-density lipoprotein) on low-density lipoprotein(LDL) and high-density lipoprotein(HDL) receptors in the hepatic stellate cell(HSC) from the rat liver. Methods HSC were isolated and cultured from the liver of Wistar rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz. Radioligand conjugation assay with 125I-LDL and 125I-HDL3 was detected for the effects of lipid on LDL and HDL receptor of HSC. Results LDL and HDL receptors were found on the membrane of the rat HSC. The lipid might increase the binding of LDL to LDL receptor, but decrease the binding of HDL3 to HDL receptor. Conclusion LDL and HDL receptors on the HSC membrane may have an important role in the metabolism of lipoprotein and the regulation of cholesterol. These results provided the basis of theory and experimentation for the genesis of fatty liver and liver fibrosis.%目的研究脂质（甘油三酯和极低密度脂蛋白）对大鼠HSC低密度脂蛋白（low-density lipoprotein，LDL）、高密度脂蛋白（high-density lipoprotein，HDL）受体的影响。方法用链霉蛋白酶和胶原酶原位灌流，Nycodenz密度梯度离心分离大鼠HSC；应用125I-LDL和125I-HDL3配体进行放射性配基结合实验测定脂质对HSC膜LDL、HDL受体的影响。结果 HSC膜表面存在LDL、HDL受体;脂质可增加LDL受体对LDL的亲合力，但降低HDL受体对HDL3的亲合力。结论 HSC膜上的 LDL、HDL受体对脂蛋白代谢及胆固醇的调节有重要作用,为阐明脂肪肝肝纤维化的发生提供了新的实验依据。