MicroRNA-200a在肝癌组织中的表达及临床意义

摘要

目的 探讨microRNA-200a (miR-200a)在肝癌组织中的表达,分析其与临床病理参数及肝癌预后的关系.方法 采用实时PCR检测肝癌细胞系和58例肝癌标本及其癌旁组织中miR-200a的表达水平,分析miR-200a表达与临床病理和肝癌患者预后的关系.结果 miR-200a在四种肝癌细胞系中表达均低于正常肝脏细胞系,在肝癌组织中表达水平(0.593±0.107)比癌旁组织(1.652±0.135)显著降低(P＜0.05);miR-200a的表达与肝癌的侵袭转移有关.有肝癌侵袭转移患者组明显低于无侵袭转移组(P=0.026).而miR-200a的表达与性别、年龄、肝炎病史、肿瘤大小、TNM分期及AFP值无关(均P＞0.05).低表达miR-200a的肝癌患者预后较差.结论 miR-200a在肝癌组织中呈低表达,并与肝癌的侵袭转移和不良预后有关.%Objective To determine the expression of microRNA-200a (miR-200a) in hepatic cancer tissues and its clinical significance.Methods The levels of expression of miR-200a in hepatic cancer cell lines,and in 58 cases of hepatic cancer and their adjacent normal liver tissues were detected by real time reverse transcription polymerase chain reaction(RT-PCR).The relations of the levels of miR-200a expression with clinicopathologic features were analyzed.Results RT-PCR revealed that the expression of miR-200a was down regulated in hepatic cancer cell lines and hepatic cancer tissues.The expression of miR200a was associated with tumor invasiveness and metastasis in patients with hepatic cancer.The expression of miR-200a in patients with tumor invasiveness and metastasis was significantly lower than those without (P ＜ 0.05).There was no significant difference with regard to sex,age,hepatitis status,tumor size,TNM staging and the level of AFP (all P ＞ 0.05).Conclusion The expression of miR-200a was downregulated in hepatic cancer tissue.There was a significant relation to tumor invasiveness and metastasis.