赤芍总苷对人黑色素瘤细胞株凋亡诱导的作用机制

摘要

Objective To investigate the influence of total paeonyglucosides (TPG)on cell apoptosis of human malignant melanoma A375 cell line and its mechanisms. Methods Human malignant melanoma cells were cultured with TPG ( 12. 5 , 25 , 50, 100, 200 mg/L) for 24, 48 h. Cell growth was detected by MTT. The cell apoptosis rate was obtained by FCM. Cells treated with 25 mg/L TPG for 48 h were collected and RT-PCR and Western blot assays were used to detect the mRNA and protein levels of Caspase-3, Bcl-2, Bax, Fas and FasL. Results TPG could inhibit the cell growth of A375. Compared with the control group, TPG significantly increased the cell apoptosis rate, and the expression of caspase-3 , Bax, Fas, FasL and decreased the expression of Bcl-2 and Bcl-2/Bax ( all P < 0. 05). Conclusions TPG induces cell apoptosis by up-regulating the caspase-3, Fas and FasL expression and decreasing the rate of Bcl-2 and Bax.%目的 观察赤芍总苷(TPG)对人黑色素瘤A375细胞凋亡的影响并探讨其机制.方法 将12.5、25、50、100、200 mg/L TPG分别加入体外培养的黑色素瘤细胞,对照组加等量生理盐水,孵育24、48 h后MTT测定细胞生长抑制率；25 mg/L TPG 孵育细胞48 h,收集细胞,FCM测定细胞凋亡率；RT-PCR和Western blot分别测定Caspase-3、Bcl-2、Bax、Fas、FasL mRNA和蛋白的表达水平.结果 TPG抑制A375细胞生长作用明显,25 mg/L TPG孵育黑色素瘤细胞48 h后,细胞凋亡率增高,Caspase-3、Bax、Fas、FasL mRNA和蛋白表达也明显升高,Bcl-2 mRNA和蛋白表达水平下降,Bcl-2/Bax比值显著降低(均P＜0.05).结论 TPG可诱导黑色素瘤肿瘤细胞凋亡,其机制与上调Caspase-3、Fas和FasL表达水平,下调Bcl-2,降低Bcl-2/Bax比值密切相关.