DNMT3B基因SNP (-149C/T)与佳木斯地区肺癌发病风险的关系

摘要

Objective To investigate the relationship between single nucleotide polymorphism C46359T of DNMT3B promoter and the susceptibility of lung cancer in Jiamusi population. Methods Genomic DNA was extracted from SO cases of lung cancer patients and 60 healthy volunteers blood Single nucleotide polvmorphism-149C/T (C46359T) of DNMT3B promoter was detected. PCR-RFLP combined with DNA sequencing technology was used. Results Compared with the control group, the person who carried the conversion C->T of SNP (-149C/T) had no increased susceptibility for lung cancer. Conclusions Single nucleotide polymorphism C46359T of DNMT3B gene may not be suitable as an independent risk factor for susceptibility to lung cancer among Jiamusi population.%目的 探讨DNMT3B基因启动子-149C/T(C46359T)单核苷酸多态性与佳木斯地区人群肺癌易感性的相关性.方法 提取50例肺癌患者及60例健康体检人员外周血基因组DNA,采用PCR-RFLP结合DNA测序技术检测DNMT3B基因启动子单核苷酸多态性-149C/T( C46359T).结果 正常对照组DNMT3B-149C/T等位基因TT/CT/CC基因型颇为97％／3％／0,病例组分别为96％／2％／2％.携带有SNP(-149C/T) C→T转换的个体患肺癌的易感性未见明显升高(x2 =0.11,P＞0.05,OR=0.83,95％CI:0.27～2.50).结论 DNMT3B基因C46359T多态性可能不适合作为佳木斯地区人群肺癌易感性的一个独立风险因素.