菩人丹超微粉对肥胖型T2DM大血管损伤大鼠ET-1、NO、FFAs含量的影响

摘要

目的 观察菩人丹超微粉(PRD)对2型糖尿病(T2DM)大血管损伤大鼠血清一氧化氯(NO)、内皮素(ET-1)、游离脂肪酸(FFAs)含量的影响,探讨PRD对肥胖型T2DM大血管损伤的保护作用及其部分机制.方法 采用雄性Wistar大鼠灌胃脂肪乳,辅以尾静脉快速注射小剂量链脲佐菌素,制备以胰岛素抵抗为特征的T2DM合并大血管病变的动物模型.成模大鼠随机分为PRD低、中、高剂量组,马来酸罗格列酮组以及模型组,各组分别灌胃给予相应药物治疗4 w,测定给药前及给药4 w末血清NO、ET-1、FFAs的含量.结果 PRD中、高剂量组均能极显著降低血清ET-1的含量(P<0.01),PRD低、中、高剂量组均能极显著提高血清NO含量(P<0.01).PRD中、高剂量组均能显著降低血清FFAs的含量(P<0.01～0.05).结论 PRD具有调节脂代谢,调整血管舒缩功能,保护损伤的血管内皮细胞的作用.%Objective To investigate effects of Pu Ren Dan(PRD) submicron powder on ET-1, NO, FFAs in large blood vessel injury rats with obesity type 2 diabetes mellitus (T2DM).To explore the protective action of PRD on large blood vessel injury with T2DM and its partial mechanisms.Methods By lavaging the male Wistar rats with fat emulsion through gastric perfusion, supplemented by rapidly injecting into tail vein with the low dose STZ, to prepare the model of obesity T2DM with large blood vessel injury charactered with insulin resistence.The model rats were randomly divided into PRD low, middle and high dose, Rosiglitazone maleate and model groups.Each group separately was treated with corresponding drugs for 4 w.The levels of NO, ET-1, FFAs were determined at the end of the 4th week.Results Compared with that of model group, the levels of ET-1 of PRD middle and high dose groups were lower significantly ( P ＜0.01 ); the levels of NO of PRD groups were lower significantly ( P ＜0.01 ); the levels of FFAs of PRD middle and high dose groups were lower obviously ( P ＜0.01 ～0.05 ).Conclusions PRD is effective in regulating lipid metabolism, adjusting vasomotoricity and protecting vascular endothelial cells in large blood vessel injury rats with obesity T2DM.