黄连素对2型糖尿病大鼠主动脉内皮功能及形态的保护作用

摘要

Objective To investigate the effects of berberine on vascular endothelial function in type 2 diabetic rats and explore its prophylactic and therapeutic significance and pharmacological mechanism.Methods Wistar rats were randomly divided into diabetic, control, diabetic rats treated with berberine ( 100 mg/kg), and control rats treated with berberine groups.The serum fasting plasma glucose (FPG), insulin, total cholesterol (TC), triglyceride (TG) levels were tested.Acetylcholine (ACh) induced endothelium-dependent relaxation and sodium nitroprusside induced endothelium-independent relaxation were measured in aortas for estimating endothelial function.In addition, histopathology measure was performed by hematoxylin and eosin staining.Results Berberine significantly decreased the levels of FPG, TC, TG in diabetic rats.Berberine also improved endothelium-dependent vasorelaxation impaired in diabetic aorta.Berberine attenuated the damage of aortic endothelium in diabetic rats.Conclusions Berberine restores diabetic endothelial dysfunction through decreasing FPG and lipid levels, improving insulin resistant and enhancing NO bioavailability.%目的 通过观察黄连素对2 型糖尿病大鼠血管内皮功能的影响,探讨黄连素对2型糖尿病血管并发症的防治意义及其机制.方法 采用小剂量链脲佐菌素腹腔注射加高脂饲料喂养的方法建立2型糖尿病大鼠模型,将模型动物随机分为模型组、黄连素治疗组,另设正常对照组以及正常黄连素组.药物干预治疗8 w后,处理动物,检测各组动物血清中空腹血糖(FPG)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、空腹血清胰岛素(fasting insulin,FINS)的水平;采用离体实验方法观察离体胸主动脉条的张力反应;HE染色观察胸主动脉形态学改变.结果 与模型组比较,黄连素组FPG、TC、TG 水平明显下降,胰岛素敏感升高(P<0.05);黄连素治疗明显改善了乙酰胆碱(acetylcholine,Ach)引起的内皮依赖性血管舒张反应,而硝普钠(sodium nitroprusside,SNP)诱导的内皮非依赖性舒张反应在各组间无显著性差异;黄连素治疗明显减轻了糖尿病引起的血管内皮细胞及血管壁病变;与正常对照组比较,正常大鼠给予黄连素灌胃,上述指标未出现明显改变.结论 黄连素对2 型糖尿病早期出现的血管内皮损伤有良好的保护作用,机制可能与其降血糖、调节血脂、改善胰岛素抵抗及内皮依赖性的血管舒张反应等作用有关.