北京市健康人群DNA聚合酶δ1的增龄性变化

摘要

Objective To investigate the change of DNA polδ1 in healthy individuals with age in Xuanwu Hospital and analyze its correlation with age.Methods The mRNA and protein level of DNA polδ1 in healthy individuals were checked by RT-PCR,Western blot.The correlation with age of ratio compared with GAPDH was analyzed.Results The mRNA and protein level of DNA polδ1 in healthy individuals were declined notably and displayed negative correlation with ages [R =0.971 , y =226.2-179.2x(mRNA); R =0.981, y =93.4238.667x(pro)].Conclusions The mRNA and protein level of DNA polδ1 in healthy individuals descends remarkably with age.%目的 探讨北京市健康人群外周血白细胞DNA聚合酶δ1表达水平的增龄性变化,建立其与增龄间的相关数学模型.方法 RT-PCR﹑Western 印迹方法测定各年龄组健康人群外周血白细胞DNA聚合酶δ1 mRNA及蛋白表达水平,将其与内参甘油醛-3-磷酸脱氢酶(GAPDH)条带灰度的扫描比值与年龄做相关分析. 结果北京健康人群DNA聚合酶δ1 mRNA及蛋白质水平随增龄显著下降,且与年龄呈直线负相关,DNA聚合酶δ1 mRNA水平与年龄的相关系数r=0.971,回归方程y=226.2-179.2x;DNA聚合酶δ1 蛋白表达水平与年龄的相关系数r=0.981,回归方程y=93.42-38.667x.结论 北京市健康人群外周血白细胞DNA聚合酶δ1水平随增龄显著下降.