低剂量辐射联合阿霉素对荷乳腺癌裸鼠肾脏组织SOD、GSH-PX和MDA的影响

摘要

目的：探讨低剂量辐射（LDR）联合阿霉素（ADM）对荷乳腺癌裸鼠肾脏组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、丙二醛（MDA）的影响。方法36只荷乳腺癌裸鼠，随机分为6个试验组（每组6只）：正常对照组，荷瘤假照组（0 mGy），LDR组（75 mGy）， ADM组，LDR-6 h-ADM组（LDR后6 h给予ADM），LDR-12 h-ADM组（LDR后12 h给予ADM）。各试验组以不同方式处理后4 d，荷乳腺癌裸鼠全部处死，取新鲜左侧肾脏组织匀浆上清液，检测SOD、GSH-PX活力、MDA 含量。结果与正常对照组相比，荷瘤假照组 SOD 和 GSH-PX 值显著降低， MDA值显著增高（P＜0.05）；与荷瘤假照组相比，ADM组SOD、GSH-PX值均明显下降，MDA值明显增高，LDR组 SOD、GSH-PX值均显著增高，MDA值显著降低（P＜0.05）；与ADM组相比，LDR＋ADM组的SOD、GSH-PX值明显增高，MDA明显降低（P＜0.05）。结论 LDR 可能增强荷乳腺癌裸鼠肾脏组织抗氧化酶活性，减少脂质过氧化产物生成，抑制氧化应激反应，从而减轻ADM对肾脏组织的损伤。%Objective To study the effects of low dose radiation ( LDR ) combined with adriamycin on superoxide dismutase ( SOD) , glutathione peroxidase ( GSH-PX) , malondialdehyde ( MDA) in kidney tissue of breast cancer-bearing nude mice.Methods Thir-ty-six breast cancer-bearing nude mice were divided into normal control, sham-irradiated(0 mGy), LDR(75 mGy), ADM and LDR-6 h-ADM, LDR-12 h-ADM groups.Take fresh kidney tissue homogenate was obtained to assay the vitality of SOD, GSH-PX and MDA con-tentResults Compared to those of normal control group, the value of SOD and GSH-PX were significantly decreased and the value of MDA was significantly increased in sham-irradiated group(P<0.05).Compared with those of sham-irradiated group, the value of SOD, GSH-PX in ADM group were decreased, while those in LDR group were increased;the value of MDA in ADM group was increased while that in LDR group was decreased(P<0.05).Compared to those of ADM group, the value of SOD and GSH-PX were increased and the value of MDA were decreased in LDR+ADM group(P<0.05).Conclusions LDR could enhance the antioxidant enzyme activity in kidney tissue of breast tumor-bearing mouse, and reduce lipid peroxidation formation producing, thereby inhibiting oxidative stress reaction to reduce doxorubicin-in-duced kidney injury.