五味子木脂素与顺铂抑制SHG-44神经球细胞的生长

摘要

Objective To explore the effect of Schisandra lignans and cisplatin on inhibiting the growth of human glioma SHG -44 neurospheres .Methods The effects of Schisandra lignans and cisplatin on the growth of SHG-44 neurospheres were detected by MTT assay . The secretion of the bcl-2 and caspase 3 protein was detected by ELISA assay .Results Schisandra lignans inhibited the growth of SHG-44 neurospheres with the concentration of 50,100,200 mg/L in a concentration-dependent manner at 24,48 and 72 h,and 10 mg/L cislatin in-hibited the growth of neuroshperes in a time-dependent manner .The secretion of bcl-2 was decreased and caspase 3 was increased in treat-ment groups .Conclusions Schisandra lignans and cisplatin could inhibit the growth of human glioma SHG -44 neurospheres .Its mechanism is correlated with down-regulation of the expression level of bcl-2 and activated caspase 3.%目的：研究五味子木脂素和顺铂对人脑胶质瘤 SHG-44神经球细胞生长的作用。方法 MTT法分析检测五味子木脂素和顺铂对SHG-44神经球细胞生长的影响；ELISA法检测细胞培养上清中bcl-2及caspase 3蛋白的变化。结果50、100、200 mg/L五味子木脂素作用24、48、72 h，均能明显地抑制SHG-44神经球细胞的生长，作用呈浓度依赖性；10 mg/L顺铂作用后也可明显抑制神经球细胞的生长，作用呈时间依赖性。 Elisa结果显示，药物作用后bcl-2蛋白水平明显下调，而caspase 3蛋白水平明显上调。结论五味子木脂素和顺铂都可以通过下调抗凋亡蛋白bcl-2的水平，进而抑制SHG-44神经球细胞的生长。