丹参注射液对 D-半乳糖致衰老小鼠学习记忆能力的影响

摘要

目的：探讨丹参对D-半乳糖致衰老模型小鼠学习记忆能力的影响及其作用途径。方法120 mg· kg－1· d－1 D-半乳糖注射6 w建立衰老小鼠模型，第15天随机分组后，丹参组注射丹参注射液0.1 ml／d连续28 d，空白对照和模型对照组注射等量的生理盐水。水迷宫实验检测小鼠的学习记忆能力；试剂盒测定血清和脑海马组织超氧化物歧化酶（ SOD）、谷胱甘肽过氧化物酶（ GSH-Px）、单胺氧化酶（ MAO）活力及丙二醛（MDA）含量。结果与对照组比较，模型组小鼠学习记忆能力降低，血清和海马组织 SOD、GSH-Px 活性下降，MDA 含量增加 MAO 活性升高（P＜0.05）；腹腔注射丹参后小鼠认知记忆能力较模型组明显改善，血清和海马组织SOD、GSH-Px活性明显升高而MDA含量减少（P＜0.05），海马中MAO活性降低（P＜0.05）。结论丹参注射液可改善D-半乳糖致衰老模型小鼠的学习记忆能力，其作用机制可能与改善血液和脑组织中 SOD、GSH-Px活力，降低血液和脑组织中MAO活性、 MDA含量有关。%Objective To study the effect of Salvia Miltiorrhiza( SM) on learning and memory ability of aging model mice induced by D-galactose.Methods D-galactose was injected 120 mg· kg-1 · d-1 for 42 days to make the aging model mice.15 d after,SM were injec-ted(0.1 ml/d)to the SM group mice for 28 days.Water maze experiment was used to detect the abilities of learning and memory of mice;su-peroxide dismutase ( SOD) ,glutathione peroxidase ( GSH-Px) ,monoamine oxidase ( MAO) activity and malondialdehyde ( MDA) content in serum and brain tissue were checked.Results Compared with that of control group,learning and memory abilities of model group was de-creased;the levels of SOD,GSH-Px activity in serum and hippocampus tissue were decreased while MAO activity and MDA content were in-creased(P<0.05).Injection of salvia miltiorrhiza obviously improved learning and memory abilities compared with those of model group;SOD,GSH-Px activity in serum and hippocampus were increased significantly and MDA content was decreased compared with those of model group(P<0.05),MAO activity in the hippocampus was also decreased(P<0.05).Conclusions Salvia Miltiorrhiza could improve learning and memory abilities of D-galactose induced aging mice through improving SOD,GSH-Px vitality and reducing MAO activity and MDA content in the blood and brain tissue.