首页> 中文期刊> 《中国老年学杂志》 >腺苷酸激活蛋白激酶与肥胖大鼠肝脏脂质沉积的关系

腺苷酸激活蛋白激酶与肥胖大鼠肝脏脂质沉积的关系

         

摘要

目的 探讨肥胖大鼠肝脏组织腺苷酸激活蛋白激酶(AMPK)的表达及其对肝脏脂质沉积的影响.方法 选用18只刚断乳的SPF级SD雄性大鼠,随机挑选6只普通饲料喂养为正常组,另一组12只高脂饲料喂养,12 w后成功建立肥胖模型大鼠6只.分别检测体重、Lee指数、内脏脂肪、肝湿重等指标.通过油红O染色和检测肝脏甘油三酯(TG)、总胆固醇(TC)含量评估两组大鼠肝脏脂质沉积的情况.采用Western印迹检测大鼠肝脏磷酸化(p)-AMPK/AMPK、p-ACC/ACC水平.结果 与正常组大鼠相比,肥胖组大鼠体重与Lee指数、附睾脂肪、肾周脂肪量、肝湿重、肝脏内TG与TC含量均显著升高,油红O染色结果显示肥胖组肝脏脂质沉积较正常组明显增多.Western印迹结果显示,与正常组大鼠比较,肥胖组大鼠肝脏p-AMPK/AMPK和p-ACC/ACC表达明显降低.两组大鼠肝脏组织中p-AMPK、p-ACC表达水平与肝脏TG、TC呈负相关.结论 高脂饮食诱导食源性肥胖大鼠可降低AMPK的活性,从而导致肝脏组织TG、TC合成增加,脂质在肝脏组织中沉积增加.%Objective To explore the expressions of AMP-activated protein kinase in the liver and the relationship with lipid deposition.Methods 18 SPF SD male rats were chosen and then randomly divided into normal (n=6) and high-fat groups (n=12).After successful modeling, 6 rats were in obese group (n=6).Rat weight, visceral fat and liver weight were compared.Lipid deposition was evaluated by oil red O dye and total cholesterol (TC) and triglyceride (TG) were measured.The expression of AMP activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in the liver were measured by Western blot.

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