增龄与运动对大鼠左心室羟基赖氨酰吡啶啉交联水平的影响

摘要

Objective To study the effects of aged and exercise training on hydroxylysylpyridinoline (HP) crosslinking concentrations of extracellular matrix in right ventricular (RV),left ventricular septum (LVS) and left ventricular free-wall (LVFW) in Fischer 344 rats.Methods We evaluated HP crosslinking concentrations of RV,LVS and LVFW extracellular matrix in young adult versus old Fischer 344 rats using high-pressure liquid chromatography (HPLC).Rats were assigned to either a sedentary control group or an exercise training group.Results HP crosslinking concentrations in LVS [(0.23±0.01) vs.(0.43±0.03)mol HP/mol Col,P＜0.05] and LVFW [(0.22±0.05) vs.(0.51±0.03) mol HP/mol Col,P＜0.05] in old sedentary control rats were elevated compared with adult sedentary rats.In adult exercise training rats,training had no effect on HP crosslinking concentration in any of the three regions of the heart.As compared with old sedentary rats,HP crosslinking concentrations in LVS [(0.22±0.06) mol HP/mol Col vs.(0.43±0.03)mol HP/mol Col,P＜0.05] and LVFW[(0.21±0.07)mol HP/mol Col vs.(0.51±0.03) mol HP/mol Col,P＜0.05] were decreased in old exercise training rats.Conclusions HP crosslinking concentration are increased with aging in LVS and LVFW of rats,and exercise can prevente this age associated change.%目的 研究增龄与运动对大鼠右心室、左心室间隔和左心室游离壁(LVFW)细胞外基质羟基赖氨酰吡啶啉(HP)交联水平的影响. 方法 采用高效液相色谱法(HPLC)检测Fischer 344青年大鼠(青年安静组和青年运动组各10只)、老年大鼠(老年安静组和运动组各10只)右心室、左心室间隔和左心室游离壁细胞外基质HP的交联水平. 结果 HP交联水平老年安静组左心室间隔和左心室游离壁分别为(0.43±0.03)mol HP/mol Col和(0.51±0.03)mol HP/mol Col,较青年安静组(0.23±0.01)mol HP/mol Col和(0.22±0.05) mol HP/mol Col升高(均P＜0.05).运动不影响青年大鼠组左心室间隔和左心室游离壁的HP交联水平.老年运动组HP交联水平左心室间隔和左心室游离壁分别为(0.22±0.06)mol HP/mol Col和(0.21±0.07)mol HP/mol Col,较老年安静组[(0.43±0.03)mol HP/mol Col和(0.51±0.03)mol HP/mol Col]降低(均P＜0.05). 结论 随着年龄的增长,大鼠左心室间隔和左心室游离壁的HP交联水平升高,运动可以阻止HP交联水平在左心室间隔和左心室游离壁的增龄性改变.