五鹤续断对阿尔茨海默病大鼠顶叶和海马β淀粉样蛋白表达的影响

摘要

Objective To study the effect of dipsacus on the expression of β-amyloid in parietal cortex and hippocampus of Alzheimer's disease model rats.Methods A total of 40 rats were randomly divided into control group,model group,dipsacus group and vitamin E group (n=10,each)General situation of rats was observed after 42 days of dipsacus treatment.The ability of learning and memory in rats was tested by Morris water maze.The content of β-amyloid in parietal cortex and hippocampus of each group rats were measured using double antibody sandwich method.Results Model group compared with control group showed that general conditions deteriorated markedly;escaping delitescence was prolonged significantly(P＜0.01) ; the activity time on the former platform quadrant reduced significantly (t=4.6261,P=0.0002) ; the number of crossing the former platform reduced significantly (t=6.5335,P=0.0000) ; the content of Aβ in parietal cortex and hippocampus increased significantly (t=4.2812,P=0.0004) (t=5.2499,P=0.0001).Dipsacus group and vitamin E group compared with model group showed that general condition improved significantly;escape latency was shortened significantly (P＜ 0.01); the activity time on the former platform quadrant increased significantly [(45.76±12.15) s,(48.70±10.25) s and (30.20±10.48) s (t=3.0666,3.9908,P=0.0066,0.0009)]; the number of crossing the former platform increased significantly [(3.02±1.19) t/2min,(3.56±0.85) t/2min and (1.43 ± 1.24) t/2min (t=2.9256,4.4804,P=0.0090,0.0003)]; the content of Aβ in parietal cortex and hippocampus reduced significantly in parietal cortex [(280.37-51.40) pg/g,(263.14 ± 45.52) pg/g versus (337.46 ±70.51) pg/g (t=2.1164,2.8003,P=0.0485,0.0118)],in hippocampus [(295.60±67.58)pg/g,(274.38±57.56)pg/g versus (388.26±83.72)pg/g(t=2.9256,4.4804,P=0.0090,0.0003)].Conclusions Dipsacus can reduce expression of Aβ in brain areas related to general intelligence that may have anti-Alzheimer's disease action.The active ingredients of dipsacus may be natural vitamin E.%目的 研究五鹤续断抗阿尔茨海默病(AD)的作用. 方法 将40只大鼠随机分为空白对照组、模型组、五鹤续断组和维生素E组,根据实验要求给药处理42 d,同时观察各组大鼠一般情况,Morris水迷宫实验检测大鼠学习记忆能力,采用双抗体夹心法测定各组大鼠顶叶皮质和海马β淀粉样蛋白(Aβ)的含量. 结果 模型组与对照组比较一般情况明显恶化逃避潜伏期均明显延长(P＜0.01),原平台象限活动时间明显减少(t=4.6261,P=0.0002),穿越原平台次数明显减少(t=6.5335,P=0.0000),大鼠顶叶皮质和海马Aβ含量显著增加(t=5.2499,P=0.0001).五鹤续断组、维生素E组分别与模型组比较:一般情况均明显改善；逃避潜伏期均明显缩短(P＜0.01)；原平台象限活动时间明显增加,分别为(45.76±12.15)s、(48.70±10.25)s和(30.20±10.48)s(t=3.0666、3.9908,P=0.0066、0.0009)；穿越原平台次数明显增多,分别为(3.02±1.19)次/2min、(3.56±0.85)次/2min和(1.43±1.24)次/2min(t=2.9256、4.4804,P=0.0090、0.0003)；大鼠顶叶皮质和海马Aβ含量显著减少,顶叶皮质分别为(280.37±51.40) pg/g、(263.14±45.52) pg/g和(337.46±70.51)pg/g(t=2.1164、2.8003,P=0.0485、0.0118),海马分别为(295.60±67.58)Pg/g、(274.38±57.56)pg/g和(388.26±83.72)Pg/g(t=2.9256、4.4804,P=0.0090、0.0003). 结论 五鹤续断可显著降低智能相关脑区Aβ的表达,具有抗AD作用,其作用的活性成分可能是其所含天然维生素E.