Objective To observe the expression of BDNF and VEGF and vasculogenesis in rats with intracerebral hemorrhage(ICH) after transplantation of NSCs. Method 140 SD rats were di-vided randomly into sham operated group(group A), model group(group B), NSCs culture medi-um transplanted group(group C) and NSCs transplanted group(group D). NSCs were transplan-ted into the subventricular zone in rat models of ICH,The expression changes of BDNF,VEGF,microvessel density(MVD) at 6 h and on days 1,4,7,14,21 and 28 after ICH was observed by means of immunohistochemical technique. Results Compared with group A, the expression of BDNF peaked on first day in groups B and C,and the expression peak was delayed to 4th day in group D,but maintained higher expression than group A on 28th day (P<0.05). At all time points,positive cell counts between group D and group B had significant difference (P<0.05).On days 4,7,14,21 and 28,positive cell counts between group D and group C had significant difference (P < 0.05). The expression levels of VEGF and MVD peaked on first day in groups B and C,and were higher in group C than in group B on days 1,4. Group D had double expression peaks on 4th day and 14th day,and maintained higher expression than in groups B,C on 28th day (P < 0.05). VEGF showed positive correlation with MVD (r=0.911 ,P<0.05). Conclusions The high expression of BDNF and VEGF is maintained for a long time and MVD increases in rats with cerebral hemorrhage after transplantation of NSCs.%目的 观察大鼠脑出血模型脑内移植神经干细胞(NSCs)后脑源性神经营养因子(BDNF)和血管内皮生长因子(VEGF)的表达及对血管生成的影响.方法 SD大鼠140只,随机分为假手术组(A组)、脑出血模型组(B组)、NSCs培养液移植组(C组),NSCs移植组(D组),每组35只.免疫组织化学方法观察各组术后6 h,1、4、7、14、21和28天BDNF、VEGF及CD34标记的微血管密度的变化,并进行相关分析.结果 与A组比较,B组、C组BDNF的表达1天达高峰(P<0.05),D组表达高峰延迟至第4天,至28天仍高于A组(P<0.05);D组在所有时间点与B组比较,差异有统计学意义(P<0.05);D组在4天后的5个时间点与C组比较,差异有统计学意义(P<0.05).B组、C组VEGF和微血管密度表达模式相同,均在1天达高峰;D组呈双高峰表达,在第4天和14天,至28天仍高于B组、C组的表达水平(P<0.05),相关分析显示,VEGF与微血管密度呈正相关(r=0.911,P<0.05).结论 脑出血大鼠脑内移植NSCs后,脑内BDNF及VEGF维持长时间的高表达,微血管密度增加.
展开▼
