Objective To study the relationship between polymorphism of lectin-like oxidized LDL receptor 1(LOX-1) gene and coronary artery disease in elderly Chinese Han population. Methods 165 subjects were divided into 2 groups according to results of coronary angiography:38 cases with normal coronary artery served as controls and 127 cases with coronary artery disease constituted coronary heart disease group. PCR single strand conformational polymorphism(PCR-SSCP)method was used to analyze LOX-1 501G/C and 3'UTR loci C/T gene polymorphism. The relationships of C genotype and T genotype with high sensitivity C-reactive protein and fibrinogen were analyzed. Results No polymorphism of Exon 4 was detected in all subjects. There was no significant difference in the 3'UTR polymorphism between patients with coronary artery disease and controls. C/T genotype could be seen at LOX-1 3'UTR. Compared with control group. The number of diseased coronary arteries and degree of coronary stenosis had no statistically significant relation with difference in frequency of 3'UTR locus C/T gene. hs-CRP and fibrinogen levels had no statistically significant difference between C genotype and T genotype. Conclusions The results show that the LOX-1 polymorphism may be not associated with the risk of coronary artery disease in the Chinese Han population.%目的 分析老年汉族冠心病患者血凝素样氧化型低密度脂蛋白1(lectin-like oxidized low-density lipoprotein receptor-1,LOX-1)501G/C和3'UTR位点C/T基因多态性与冠状动脉病变支数及狭窄程度的关系.方法 选择行冠状动脉造影患者165例,根据冠状动脉造影结果分为冠状动脉正常对照组(对照组)38例,冠心病组127例.采用PCR单链构象多态PCR-SSCP法分析LOX-1 501G/C和3'UTR位点C/T基因多态性.分析C基因型、T基因型与患者高敏C反应蛋白、纤维蛋白原的关系.结果 LOX-1第4外显子501G/C位点未见多态性表现.LOX-1 3'UTR位点可见C/T基因型.与对照组比较,冠心病组患者冠状动脉病变支数及冠状动脉狡窄程度均与3'UTR位点C/T基因发生频率,差异无统计学意义(P＞0.05).高敏C反应蛋白和纤维蛋白原水平在3'UTR位点C基因型与T基因型中差异无统计学意义(P＞0.05).结论 LOX-1基因多态性与汉族冠心病发病无关.
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