大鼠眶下孔注射高渗盐水阿霉素后三叉神经节的病理变化

摘要

目的：本实验旨在研究大鼠眶下孔注射高渗盐水阿霉素后，三叉神经功能和三叉神经节细胞病理形态的变化。方法：48只SD大鼠，随机分为A、 B两组，每组24只，每组又分四个时间点：1周组，2周组，1月组，3月组取材，每时间点6只大鼠。将A、 B组分别给予10％NaCl和1％阿霉素的混合溶液10μl眶下孔注射，生理盐水10μl眶下孔注射(B组)每组给药后于1周、2周、1月及3月时测试各组剩余大鼠的触须垫感觉功能，后处死取三叉神经节标本，制作HE染色切片，分别观察神经节细胞的形态学变化，用等距随机抽样法记录节细胞总数及异常节细胞率。结果：各组动物感觉功能测试， A、 B组同时间点有统计学差异(P<0.01)，眶下孔内神经周围注射高渗盐水阿霉素溶液后A1-A4组节细胞总数呈递减趋势(同组各时间点分别比较， P<0.01)，显著低于B组同时间点(P<0.01)，而与B组各时间点相比，无统计学差异(P>0.05)；异常节细胞率A1-A4组呈递增趋势(A组各时间点相比(P<0.01)，显著高于B组同时间点(P<0.01)。结论：大鼠眶下孔内神经周围注射高渗盐水阿霉素后，阿霉素可在高渗盐水的作用下被神经纤维大量吸收并进一步逆行运输到三叉神经节，随着时间的延续引起三叉神经节细胞的渐进性退变。%Objective: The injection with compound solution of 10% NaCl and 1% adriamycin wasperformed in SD rats , tocompare the changes in function of trigeminal nerve and pathomorphology of trigeminal ganglion cells at different time points. To investigate the feasibility of treatmentof trigeminal neuralgia by perineural (infraorbital hole) injection with mixed solution of hypertonic saline and adriamycin. Methods: 48 SD rats were randomly allocated into two roups (A, B group), with 24 rats in each group. Either receiced 10ul compound solution of 10% NaCl and 1% Adriamycin (group A) or physiological saline (group B) alone were injected from the infraorbital foramen. Each group was then divided into four subgroup: 1-week subgroup, 2-week subgroup, one month subgroup, 3 month subgroup subjects, and each subgroup had six rats.At 1th week, 2th week, 1th month, 3th month after operation, the remaining rats’ sensation of the vibrissae of pad was tested, and the gasseringganglions were took out to observe the total number of ganglions cells, the ratio of abnormal ganglions cells and cells’ appearance. Results: Significant difference was found in intercomparison of rats’ sensation of the vibrissae of pad of the subgroups at the same timepoint between group A and B (P<0.01) After 10μl compound solution of 10% NaCl and 1% adriamycin injection from the infraorbital foramen, a decreasing tendency of the total number of ganglions cells was observed from subgroup 1 to 4 in group A (P<0.01), lower than that at the same timepoint in group B (P<0.01) While an increasing tendency of the ratio of abnormal ganglion cells was observed from subgroup 1 to 4 in group A (P<0.01), higher than that at the same timepoint in group B (P<0.01) No statistical difference was found among subgroups in group B. Conclusion: After 10ul compound solution of 10% NaCl and 1% adriamycin injection from the infraorbital foramen of rats, hypertonic saline maycontribute to enhancing axoplasmic transport of adriamycin, leading tonecrosis of trigeminal ganglion cells. This method could probably provide a novel and effective treatment for trigeminal neuralgia.