Objective To investigate the mechanism of adiponectin on HSC-T6 cells migration and estimate the function of the NO during the procedure. Methods HSC-T6 cells were divided into 3 groups. HSC-T6 cells were treated by adiponectin, qPCR and Western blot were used to investigate the expression of iNOS gene and protein respectively. Scratch-wound healing method was used for migration investigation. Results In group treated with adiponeetin, the expression of iNOS gene and protein increased significantly ( P ＜ 0.01 ). The nitrateitrite concentration in HSC-T6 cell culture medium increased (P ＜0.01 ). The HSC-T6 cells migration was inhibited significantly. Conclusion Adiponectin could inhibit HSC-T6 cells migration, which could be mediated by NO partially at least.%目的 探讨脂联素对肝星状细胞-T6细胞系(hepatic stellate cell-T6 cell line, HSC-T6)细胞迁移作用机制,并评价一氧化氮(nitric oxide, NO)在此过程中的作用.方法 以脂联素治疗肝HSC-T6细胞,采用qPCR检测肝脏星状细胞的iNOS基因表达水平;Western blot法检测iNOS蛋白的表达以及划痕修复实验检测脂联素作用下HSC-T6的迁移情况.结果 脂联素作用下,iNOS基因及蛋白表达均明显升高,HSC-T6细胞培养基中NO代谢产物浓度显著升高,HSC-T6细胞迁移显著受抑制.结论 脂联素具有抑制HSC-T6细胞迁移的作用,这种作用可能是通过NO介导实现的.
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