白斑狗鱼家系亲本遗传结构的微卫星分析

摘要

近年来，新疆土著鱼类白斑狗鱼（Esox lucius）已成为重要的养殖对象。本研究用8对微卫星标记扩增了190~360bp的2~7条带，分析构建了白斑狗鱼家系亲本的遗传结构。白斑狗鱼雌、雄亲鱼的观测杂合度和预期杂合度分别为0.6438、0.7066和0.6500、0.7076,表明白斑狗鱼亲本杂合度较高。雌、雄亲鱼平均多态信息含量分别为0.6464和0.6486。总体上8个微卫星位点具有较高的多态性，雌雄亲本间遗传距离为0.0426，适于分析白斑狗鱼亲本的遗传结构。F统计显示，白斑狗鱼近亲交配不严重，适合作为家系选育亲本。%NorthernpikeEsox luciusasanendemicfishinXinjianghasbeenanimportantcandidateforaquacultureinrecentyears.In this study, eight pairs of m icrosatellite m arkers w ere used to analyze the genetic structure of broodstock fishes to construct northern pike fam ilies.T he am plification bands w ere found to be ranged from 2 to 7,and the products w ere varied from 190 bp to 360 bp size. The fem ale broodstock had observed heterozygosity of0.6438,and expected heterozygosity of0.7066 and the m ale ones show ed ob-served heterozygosity of0.6500,and expected heterozygosity of0.7076,indicating thatnorthern pike had high heterozygosity.The PIC values w ere found 0.6464 in fem ale parents and 0.6486 in m ale parents.T he results show ed thatthere w as high polym orphism in 8 m i-crosatelliteslocionthewhole,whichwassuitableforgeneticstructureanalysis.Thegeneticdistanceof0.0426wasobservedbetween fem ale and m ale broodstocks,and no serious inbreeding w as show n by F statistics in the parents w hich w ere suitable for fam ily con-struction.