目的:探讨玻璃化冷冻法在未成熟卵子冷冻方面的应用价值.方法:选取GV期卵母细胞744枚,随机分为5组,A组(128枚),GV期卵子直接进行未成熟卵母细胞体外培养(IVM);B组(220枚),GV期卵子直接行玻璃化冷冻;C组(155枚),GV期卵子直接行IVM,待培养至MⅡ期,再行玻璃化冷冻;D组(112枚),GV期卵子去除冠-丘细胞,行玻璃化冷冻;E组(129枚),GV期卵子保留除冠-丘细胞行玻璃化冷冻.比较各组卵母细胞的存活率、成熟率、受精率、卵裂率及囊胚形成率.结果:与A组相比,B组和C组的成熟率、受精率、卵裂率和囊胚形成率均降低(P＜0.05);B组与C组间存活率、成熟率、受精率、卵裂率和囊胚形成率无差异(P＞0.05).E组的成熟卵子率较D组提高(P＜0.05),但两组在解冻卵子存活率、受精率、2细胞数和＞2细胞数方面无差异(P＞0.05).结论:未成熟卵子的玻璃化冷冻保留冠-丘细胞可显著提高冻融效果.%Objective: To study tfce outcomes of different vitrification protocols for the cryopreservation of human immature oo-cytes. Methods: A total of 744 spared oocytes in GV stage were collected and randomly allocated into 5 groups. Group A ( n = 128) : directly for in vitro maturation (IVM) ; Group B (n =220) : vitrified without IVM; Group C (n = 155) : IVM to M Ⅱ stage and then vitrified; Group D ( n = 112) ; vitrified after crown - cumulus cells removed; Group E ( n = 129) : vitrified with presence of crown - cumulus cells. The rates of survival, maturation, fertilization, cleavage and blastocyst formation of the frozen - thawed/fresh GV oocytes were compared between the groups. Results: Compared with those in Group A, the rates of maturation, fertility, cleavage and blastocyst formation in Group B or Group C were significantly lower (P < 0.05). There was no significant difference between Group B and Group C in survival, maturation, fertilization, cleavage, blastocyst formation rate (P > 0.05 ). The rate of maturation in Group E was higher than that of Group D (P < 0.05) , but there was no significant difference between these two groups in survival rate, fertilization rate, number of 2 - cell embryos or > 2 - cell embryos ( P > 0. 05 ) . Conclusion: Vitrification of human immature oocytes with intact crown - cumulus cells can significantly improve the cryopreservation outcomes.
展开▼
