微柱凝胶法和传统试管法在新生儿溶血病诊断中的比较研究

摘要

目的:探讨微柱凝胶技术对新生儿溶血病(HDN)的诊断价值.方法:选取128例疑似HDN的新生儿,分别用微柱凝胶法和传统试管法进行直接抗人球蛋白试验(DAT)、间接抗人球蛋白试验(IAT)和放散试验并对比结果.结果:在128例中104例诊断为HDN患儿,微柱凝胶法的阳性检出率为92.31％,传统试管法为80.77％,微柱凝胶法的敏感性高于传统试管法(x2=5.92,P＜0.05)；在微柱凝胶法3项试验中,以放散试验的阳性率最高,DAT阳性率最低.结论:对于辅助诊断HDN,微柱凝胶法比传统试管法更具优势.%Objective: To explore the value of the microcolumn gel technology in diagnosing hemolytic disease of the newborn. Methods: The direct antiglobulin test ( DAT) , indirect antiglobulin test (IAT) and elution teat were conducted with the micro-column gel technology and the traditional method in 128 newborns suspected hemolytic disease of the newborn. Results; In 104 newborns with clinical diagnosis of hemolytic disease of the newborn, the positive rates of the microcolumn gel technology and the traditional method were 92.31% and 80.77% (x2 =5. 92,P<0.05). As for three methods with the microcolumn gel technology, the positive rate of the elution test was the highest and that of DAT was the lowest. Conclusion: A microcolumn gel technology is better for assistant diagnosis of hemolytic disease of the newborn when compared with the traditional method.