共刺激分子B7-H4在宫颈癌组织中的表达及其临床意义

摘要

目的 探讨宫颈癌组织中B7-H4的表达水平及临床意义.方法 选取哈尔滨医科大学附属第一医院2010~2012年因子宫肌瘤行子宫全切的正常宫颈组织30例,宫颈活检及LEEP刀治疗的宫颈上皮内瘤变组织30例及宫颈癌患者的宫颈癌组织40例,采用PV-二步法免疫组织化学方法检测新型共刺激分子B7-H4在各组中的表达情况.结果 B7-H4在宫颈癌组织中较宫颈上皮内瘤变组织中和正常宫颈组织中表达的阳性率高,差异有统计学意义(P<0.05);在宫颈上皮内瘤变组织中与正常宫颈组织中表达阳性率比较,差异无统计学意义(P>0.05).B 7-H 4的阳性表达率随着宫颈癌患者病理分级的升高,淋巴结转移,呈增高趋势,但与患者年龄无关.结论 B7-H4参与了宫颈癌的发生发展,有望成为宫颈癌新的诊断及治疗指标.%Objective To investigate the expression of B7-H4 in cervical cancer and its clinical significance. Methods A total of 30 cases of normal cervical tissue with hysterectomy,30 cases of cervical intraepithelial neoplasia treated by cervical biopsy and LEEP knife,and 40 cases of cervical cancer tissue were selected in the First Affiliated Hospital of Harbin Medical University from 2010 to 2012,the expression of B7-H4 was detected by PV-2-step immunohistochemistry. Results The positive expression rate of B7-H4 in cervical cancer group was higher than that of the cervical intraepithelial neoplasia group and normal cervical tissue group,the differences were statistically significant(P<0.05);there was no significant difference in the positive rate of cervical intraepithelial neoplasia compared with normal cervical tissues(P>0.05). The positive expression rate of B7-H4 increased with the pathological grade of cervical cancer,lymph node metastasis,but had no correlation with the age of patients. Conclusion B7-H4 is involved in the occurrence and development of cervical cancer, and is expected to become a new diagnostic and treatment indicator of cervical cancer.