胶质细胞源性神经营养因子对运动神经再生的研究

摘要

目的 观察局部应用胶质细胞源性神经营养因子(GDNF)对于大鼠运动神经再生效果的影响.方法 SD大鼠30只,造成股神经一侧缺损5 mm,另一侧股神经作为正常对照侧;缺损段以硅胶管套接后将硅胶管与神经外膜缝合固定,然后随机分为两组:一组硅胶管内注入GDNF,另一组注入等量生理盐水.术后4周取材,进行溃变神经轴突染色和辣根过氧化物酶(HRP)逆行标记追踪,求出修复侧标记胞体数占正常侧标记胞体的百分率,评价股神经的再生状态.结果 与生理盐水组比较,GDNF可以缓解损伤神经的近侧端纤维发生溃变,促进更多的前角运动神经元轴突再生.结论 股神经损伤后应用外源性GDNF可促进股神经再生.%Objective To research the effect of glial cell line-derived neurotrophic factor (GDNF) on rat motor nerve regeneration following injury.Methods 30 SD rats were randomly divided into two groups:Defect of 5 mm was made in one side of femoral nerve in 30 rats.Silica tubes were used to bridge the defects.GDNF was injected into silicone tube within a group,another group was injected with saline as the control.4 weeks after operation,degeneration of axons were stained to calculates the repaired side nerve with the normal side of the area percentage of fiber degeneration.At the same time,horseradish peroxidase (HRP) retrograde tracing mark was used to calculates the percentage of marked cell body.Results Compared with the control group,GDNF can relieve nerve damage occurs proximal end of the fiber degeneration and promote more anterior horn neurons axon regeneration.Conclusion GDNF can enhance the degeneration of femoral nerve injured.