新鲜羊膜移植在碱烧伤角膜治疗中的抗炎及抗氧化作用

摘要

目的研究新鲜羊膜移植在早期角膜碱烧伤治疗中的抑制炎症作用及其与自由基反应的关系。方法以32只新西兰大白兔制作角膜碱烧伤模型，烧伤后24 h行新鲜羊膜移植手术，并设空白对照组。分别于术后14，30天处死实验兔。以计算机图像分析系统测定角膜中多形核白细胞（PMN）含量。并检测同时期角膜中超氧化物歧化酶（SOD）活力及丙二醛（MDA）含量。结果新鲜羊膜移植组角膜PMN含量显著少于对照组；其MDA含量低于对照组，SOD活力显著高于对照组。结论新鲜羊膜移植在早期角膜碱烧伤治疗中能抑制角膜内急性炎症反应，减轻自由基反应对角膜组织造成的损害。%ObjectiveTo understand the effect of fresh amniotic membrane transplantation on restraining inflammatory response and its relationship with free redical reaction in the treatment of early corneal alkali burn．MethodsCorneal alkali-burn models of 32 New Zealand white rabbits were made．24 hours after burn，the fresh amniotic membrane transplantation （AMT） were adopted in one group，and another group were as control （C）．14 days and 30 days after operation，the contents of polymorphonuclear leukocyte （PMN） in corneas by using computer image analysis were measured，and the activity of superoxide dismutase （SOD） and the level of malondialdehyde （MDA） were determined at the same time．ResultsThe contents of PMN in AMT were lower than that in C（P＜0.01）．The activity of SOD was higher and the level of MDA was lower than that in C．ConclusionFresh amniotic membrane transplantation can restrain the acute inflammatory infiltration in alkali-burn corneas and reduce the damage of free redical reaction to the corneas．