组织因子在脉络膜黑色素瘤中的表达及意义

摘要

Background It is well known that tissue factor (TF) is expressed in tumor cells and neovascular endothelial cells of tumor.It plays an important role in the formation of new blood vessels as well as the growth and metastasis of tumor.However,whether TF is expressed or not in choroidal melanoma(CM)is unclear.Objective This study is to investigate the expression of TF in a choroidal melanoma cell line and human choroidal melanoma.Methods The expression of TF was studied in the optimal choroidal melanoma-1 (OCM-1) cell line and ten specimens from CM patients using immunhistochemistry.Ten normal eye specimens from donators were used as controls.Results The TF protein was mainly expressed in the cytoplasm.It is over-expressed in OCM-1 cells with positive rate of 85.33±5.47%.Hyper-expression of TF also was found in human choroidal melanoma with a positive rate of 41.60±14.17%.The integrated optical density (IOD) of positive cells in CM was 33853.67±16445.30,and only 5.65±4.26% of positive cells was found in normal human choroidal tissue.The IOD of TF expression in normal human choroidal tissue was 426.43±316.62.Conclusion The overexpression of TF in CM cells may be a new immunotherapy target for CM treatment.%目的 探讨组织因子(TF)在脉络膜黑色素瘤(CM)细胞株及人CM组织中的表达,为CM的治疗提供新的思路和方法 .方法 体外培养人CM细胞株(OCM-1),应用免疫组织化学染色法观察TF在OCM-1中的表达.对人CM眼球标本行免疫组织化学染色检测TF在CM组织中的表达.结果 OCM-1细胞中TF蛋白主要表达在细胞质,阳性细胞率为(85.33±5.47)%.CM组织中TF蛋白主要表达在细胞质,少部分表达于CM细胞膜及CM组织内血管内皮细胞的细胞质.CM组织切片中,TF表达阳性细胞率为(41.60±14.17)%,正常人眼脉络膜组织中TF表达阳性细胞率为(5.65±4.26)%,差异有统计学意义(t=5.433,P＜0.01).CM组织切片阳性细胞积分光密度(IOD)值为33853.67±16445.30,正常人眼脉络膜组织切片平均IOD值为426.43±316.62,差异有统计学意义(t=4.460,P＜0.01).结论 TF在OCM-1细胞及CM组织中呈特异性表达,可作为CM的一个特异性免疫治疗靶点,为CM的治疗提供了新的思路.