阿司匹林对萘性白内障α-晶状体蛋白分子伴侣活性的保护作用

摘要

目的 探讨低剂量阿司匹林对萘性白内障α-晶状体蛋白分子伴侣活性的保护作用.方法 将45只150～160g雌性SD大鼠随机分为空白对照组(无特殊处理)、萘性白内障组(每日0.5mg/kg萘灌胃3d后改为每日1mg/kg)和阿司匹林组(萘灌胃4h后每日100mg/kg阿司匹林灌胃).应用定期图像分析和高效液相色谱(HPLC)法分离纯化α-晶状体蛋白,紫外分光光度法测定其抑制热诱导的β-L晶状体蛋白变性的能力.结果 3周时萘性白内障组晶状体开始出现混浊,程度逐步加重,阿司匹林组混浊早期进展较萘性白内障慢,6周后进展程度接近于萘性白内障组.实验2周时3组晶状体混浊程度的比较差异无统计学意义(F=0.032,P=0.969).实验第4、6、8、10周时3组晶状体混浊程度比较差异均有统计学意义(F=5031.130,P=0.000;F=115964.000,P=0.000;F=169846.500,P=0.000;F=195431.200,P=0.000),且空白对照组与萘性白内障组、空白对照组与阿司匹林组、阿司匹林组与萘性白内障组之间的差异均有统计学意义(P=0.000).阿司匹林组的α-晶状体蛋白分子伴侣活性高于萘性白内障组.结论 低剂量阿司匹林通过保护α-晶状体蛋白分子伴侣活性延缓萘性白内障大鼠晶状体混浊的进展,此作用在白内障早期尤为明显.%Background Age-related cataract is the leading cause of visual impairment worldwide.To seek the effective prevention and drugs for management of cataract is important.Naphthalene-induced cataract of rat is an ideal animal model for the research of human age-related cataract,and aspirin has been proven to inhibit the development of human age-related cataract.ObjectiveThe present study is to investigate the role of aspirin on naphthalene-induced cataract.Methods Forty-five 150-160 g female SD rats were divided into three groups randomly.Naphthalene was orally taken with 0.5mg/kg per day for 3 days and then 1mg/kg per day for 70 days,and then 100mg/kg of aspirin was given per day for 70 days following four-day washout period in group A.In group B,the animals was given orally only naphthalene at the same way.No any intervention was used in group C.Naphthalene-induced cataract was examined under the slim lamp every week.The experimental animals were sacrificed and lenses were obtained in 70 days.α-Crystalline was extracted from lens homogenate and purified and identified using High Performance Liquid Chromatography(HPLC),2-dimentional electrophoresis gel and Western blot.Different abilities of α-crystalline to protect β low crystalline from aggregation were observed using ultraviolet spectrophotometer.Results Naphthalene-induced cataract formed at the third week in only naphthalene group but at the sixth week in naphthalene+aspirin group under the slim lamp.No significant difference was found in the degree of lenses opacity in the second week among these three groups(F=0.032,P=0.969).However,a statistically significant difference was seen in the degree of lenses opacity in the fourth,sixth,eighth and tenth week among these three groups(F= 5031.130,P=0.000;F=115964.000,P=0.000;F=169846.500,P=0.000;F= 195431.200,P=0.000).Themolecular chaperone-like activity was significantly higher than that of the naphthalene-induced group.Conclusion Aspirin delays the progression of lens opacification through protecting α-crystalline molecular chaperone-like activity.