Bacterial strain for expression of recombinant protein having DEGP genes deficient in protease that retains chaperone activity and TSP and PTR genically deactivated

摘要

A recombinant gram-negative bacterial cell comprising: a. a mutated Tsp gene, in which the mutated Tsp gene encodes a Tsp protein that has 50% or less of the protease activity of a wild-type non-mutated Tsp or is a Tsp gene mutated by gene deactivation; wherein the cell is isogenic of a E. coli W3110 cell, except for the mutated Tsp gene and optionally a polynucleotide sequence encoding a protein of interest.