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BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES
BACTERIAL STRAIN FOR RECOMBINANT PROTEIN EXPRESSION, HAVING PROTEASE DEFICIENT DEGP RETAINING CHAPERONE ACTIVITY, AND KNOCKED OUT TSP AND PTR GENES
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机译:用于重组蛋白表达,具有蛋白酶缺陷的DEGP保持伴侣活性,并剔除TSP和PTR基因的细菌菌株
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摘要
A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity ; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest.
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