Prokaryotic Expression of Infectious Bronchitis Virus S1 Gene and Analysis of Biological Activity of Recombinant Protein

摘要

[Objective] To study the prokaryotic expression and antigenicity identification of S1 gene from avian infectious bronchitis virus(IBV).[Method] The S1 gene was cloned into a pMD18-T vector to yield a recombinant plasmids pMD18-T-IBV-S1.Then S1 gene was inserted into the multiple cloning site of a prokaryotic expression vector pET-32a(+).The recombinant plasmid was transformed into E.coli BL21.The recombinant protein was induced by IPTG and measured by SDS-PAGE and western-blotting.[Result] The S1 gene was successfully expressed in E.coli BL21,the fusion proteins were about 66.0 kDa in a form of inclusion body.Western-blotting test showed that the recombinant proteins could be identified by IBV polyclonal antibody.[Conclusion] The recombinant proteins of S1 gene have the antigenicity,which lays a good foundation for further research on new generation vaccine of IBV.