Background The main mechanism of diabetic retinopathy (DR) is the formation of retinal neovascularization. Vascular endothelial growth factors (VEGF) is primarily factor for DR. Objective The goal of this study was to investigate the effects of angelicae sinensis decoction for supplementing blood ( ASD), a kind of traditional prescription of Chinese herbal medicine, on vascular endothelial growth factor (VEGF) level in vitreous and serum of STZ-induced type 2 diabetes mellitus. Methods Thirty-four SPF male SD rats were fed using high fat diet for 8 weeks and then the streptozocin of 25 mg/kg was intraperitoneally injected to create the type 2 diabetic models. ASD (3.6 g/kg) and 10 mg/kg Gli of 5 mi were administered intragastrically from the 4th day of high fat die though 8 weeks respectively. Other 10 matched rats were used as normal control group. The body weight, blood glucose level ,triglycerides (TG) and high density lipoprotein (HDL) were detected in 4 and 8 weeks after usage of drug. The vitreous and serum samples were obtained in the 8 th weeks for the VEGF detection by enzyme-linked immunosorbent assay (ELISA). This experiment comlied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results The body weight and HDL in DM group were significantly lower than those of normal control group in 4 or 8 weeks ( P＜0. 01 ), but in ASD and Gli groups ,the body weight and HDL were higher than those of the DM group (P＜0. 05 ). the blood glucose level and TG were elevated in DM group compared with normal control group (P＜0. 01 ), but those in ASD group and Gli group were decreased in comparison with DM group ( P＜0.05 ). EIISA revealed that no significant differences were found in VEGF levels between normal control group and ASD group or Gli group (P＞0. 05 ), but in DM group, the serum VEGF level (147.65±4. 55 ng/L) was reduced and vitreous VEGF level (673.45± 100. 85 ng/L) was raised,showing the considerable differences (P＜ 0.05 ). No any correlation was found between serum VEGF level and vitreous VEGF level (r=0.314,P＞0. 05). Conclusion ASD can decrease the VEGF level in vitreous,implying that ASD postpone the occurrence of diabetic retinopathy in DM rat.%背景 糖尿病视网膜病变(DR)主要的病理改变是新生血管生成,血管内皮生长因子(VEGF)是诱导新生血管生成的重要因子,因此是评价糖尿病(DM)血管性疾病并发症的重要指标.目的 研究当归补血汤(ASD)对链脲佐菌素(STZ)诱导的DM大鼠玻璃体和血清中VEGF表达的影响,探讨其在DR治疗中的作用机制.方法 雄性SPF级SD大鼠34只以高脂饮食喂养8周,然后腹腔注射小剂量(25 mg/kg)STZ建立2型DM大鼠模型,将血糖＞13.9 mmol/L者纳入实验并用计算机数字随机生成法随机分组.11只DM动物作为模型组,ASD治疗组10只、格列奇特治疗组(Gli组)10只,于造模第4天开始分别以3.6g/kg ASD、10 mg/kg Gli(5 ml)和饮用水灌胃至实验结束.另取10只正常匹配动物饲以正常饲料作为正常对照组,以等量饮用水灌胃,分别于灌胃后第4、第8周收集各组动物腹主动脉血检测血糖、血甘油三酯(TG)、高密度脂蛋白(HDL),灌胃后第8周采用酶联免疫吸附试验(ELISA)测定大鼠血清和玻璃体中VEGF的质量浓度.结果 灌胃治疗后第4周、第8周,ASD组血糖和TG明显低于DM组,差异有统计学意义(P＜0.05);HDL高于DM组,差异有统计学意义(P＜0.05);灌胃后第8周,正常对照组、ASD组、Gli组间血清、玻璃体中VEGF质量浓度的差异均无统计学意义(P＞0.05);而DM组大鼠血清中VEGF质量浓度明显低于正常对照组,玻璃体中VEGF质量浓度明显高于正常对照组,差异均有统计学意义(P＜0.01);DM组大鼠血清中和玻璃体中VEGF质量浓度分别为(147.65±4.55)和(673.45±100.85)ng/L,二者间无明显直线相关关系(r=0.314,P＞0.05).结论 结果提示ASD能够降低玻璃体中VEGF的质量浓度,可能有延缓DM大鼠DR的发生的作用.
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