CpG ODN激活慢性HBV感染者外周血单个核细胞和CD4+T细胞功能的研究

摘要

Objective To study the effects of CpG oligodeoxyribonuclentide (ODN)as adjuvant on the immune responses in PBMC and CD4+ T cell with chronic hepatitis B virus. Methods The selected 20 infections were averagely divided two groups. The frequency of IFN-γ secreting PBMC and CD4+ T cell in immune tolerant phase and in the immune clearance phase that had stimulated by CpG ODN, HBsAg and Mixture[CpG ODN+HBsAg] were analyzed by enzyme linked immune spot(ELISOT). Results The PBMC and CD4+ T cell were differently incubated by CpG ODN+ HBsAg and M[CpG ODN + HBsAg]. The number of IFN-γ spot differently are 3±8, 339±429, 375±496, 1±4, 5±16 and 5±12;the results of immume tolerance are 3±8,361±153, 375±276, 0±2, 2±2 and 4±4; but the results of immune clearance are 3±21, 289±345, 405±656, 2±14, 8±40 and 7±30. The IFN-γ spots statistical analysis of PBMC were differently incubated by HBsAg and M,the total is P = 0.720, The IFN-γ spots statistical analysis of CD4+ T cell were differently incubated by HBsAg and M, the total is P = 0. 890, The IFN-γ spots statistical analysis of PBMC and CD4+ T cell were differently incubated by M, the total is P=0.000. Conclusions The ability that CpG ODN can not significantly increase the IFN-γsecreting of PBMC and CD4+ T cell that were incubated by HBsAg to the infection in immune tolerant phase and in the immune clearance phase, but the PBMC outweighed The CD4+ T cell.%目的 探讨人工合成硫代修饰的含CpG基序的寡脱氧核苷酸(ODN)作为佐剂对慢性HBV感染者PBMC和CD4+T细胞产生免疫应答的影响.方法 将入选慢性HBV感染者20例分成两组,免疫耐受组和免疫清除组各10例,用CoG ODN、HBsAg、M[CpG ODN+HBsAg]分别与同一感染者的PBMC和CD4+T细胞孵育,采用酶联免疫斑点法(ELISPOT)检测两种细胞IFN-γ的分泌情况,以斑点数的多少来评价细胞分泌IFN-γ的能力.结果 CpG ODN、HBsAg、MECpG ODN+HBsAg]与PBMC孵育后IFN-γ斑点数是3±8、339±429、375±496;与CD4+Tcell孵育后IFN-γ斑点数是1±4、5±16、5±12.在免疫耐受组,CpG ODN、HBsAg、M[CpG ODN+HBsAg]与PBMC孵育后IFN-γ斑点数是3±8、361±153、375±276;与CD4+ Tcell孵育后IFN-γ斑点数是0±2、2±2、4±4.在免疫清除组,CoG ODN、HBsAg、M[CoGODN+HBsAg]与PBMC孵育后IFN-γ斑点数是3±21、289±345、405±656;与CD4+ Tcell孵育后IFN-γ斑点数是2±14、8±40、7±30.PBMC与HBsAg、M孵育后IFN-γ斑点数比较统计分析P=0.720;CD4+ Tcell与HBsAg、M孵育后IFN-γ斑点数统计分析P=0.890,两种不同细胞中与M孵育后IFN-γ斑点数比较统计分析得P=0.000.结论 在免疫耐受组和免疫清除组,CpG ODN不显著增加HBsAg刺激PBMC和CD4+ T细胞分泌IFN-γ,但CpG ODN对PBMC刺激效果优于CD4+T细胞.