南京95例肝病患者中3种人细小病毒感染的检测与分析

摘要

目的 建立人细小病毒B19、人博卡病毒(HBoV)及人细小病毒4(PARV4)分子检测方法,并应用于南京95例肝病患者血浆中三种人细小病毒的感染情况分析.方法 分别建立人细小病毒B19、HBoV及PARV4感染的巢式PCR方法,确定其灵敏度与特异性；并对南京市某医院95例住院肝病患者血浆中三种人细小病毒的感染情况进行检测,并对检测结果进行统计学分析.结果 建立的三种巢式PCR方法特异性好,检测灵敏度均可达10个拷贝.95例肝病患者血浆中检出B19感染2例(2.1％),HBoV感染9例(9.5％),且发现在5例药物性肝炎患者中检出HBoV阳性3例,但未检出PARV4.结论 人细小病毒B19、人博卡病毒(HBoV)及人细小病毒4(PARV4)巢式PCR检测方法灵敏特异.从肝病患者血浆中检出了B19及HBoV.%Objective To analyze the infection of human parvovirus B19,human bocavirus(HBoV) and human parvovirus 4 (PARV4) in blood samples among patients with liver disease in Nanjing by molecular detection.Methods Nested PCR assays were designed and validated to detect B19,HBoV and PARV4,respectively.The assays were used to screen three parvoviruses in blood samples from 95 patients with different liver disease in Nanjing.The parvovirus infection was analyzed statistically.Results The detection limits were 10 copies of genomic DNA equivalents per reaction for each assays and the good specificity were observed.The frequency of B19 and HBoV were 2/95 (2.1％)and 9/95 (9.5％)in blood samples respectively.No PARV4 was detected.HBoV was detected in 3/5 patients with drug-induced hepatitis.Conclusion Both B19 and HBoV infection were detected in blood from patients with liver disease.