2015年甘肃省外环境禽流感病毒监测预警分析

摘要

Objective To understand the contamination status of avian influenza virus H5,H7,and H9 in environmental samples in Gansu province during 2015,and provide scientific guidance for the prevention and control of human infection with AIV in the province.Methods We detected the AIV nucleic acid of 2015 environmental samples with real-time PCR;the samples were collected from all of the monitoring points in the whole province,then we analyzed the result of environmental monitoring of pathogens in the province.Results The detection rates of FluA,H5,H7 and H9 were 19.90％,0.05％,0 and 19.65％ respectively.The sample specific detection rate of FluA was the highest in wipe specimen of the board surface of slaughtering or placing poultry (31.09％) and the lowest in stool specimens (13.85％).FluA detection rate was the highest in December and the lowest in June and July (8.59％ and 8.66％).The detection rate of Dingxi city and Longnan city of nucleic acid of avian 2influenza virus FluA and H9 were significantly higher than those in other cities in the province(x =4.523～ 88.059,P ＜ 0.05).Conclusions The main AIV pollution of the external environment in Gansu province in 2015 were the H9 subtype,and there was no H7N9 positive,we should strengthen the monitoring and early warning for avian influenza.%目的 了解甘肃省外环境中禽流感病毒H5、H7、H9亚型的分布状况,科学指导全省禽流感防控工作.方法 采用实时荧光定量聚合酶链反应法(Real-time fluorescence quantitativePolymerase Chain Reaction,real-time PCR)对2015年全省14个市州采集的标本2 015份进行禽流感病毒核酸检测.结果 FluA、H5、H7、H9的检出率依次为:19.90％、0.05％、0和19.65％.FluA检出率最高的环境标本为宰杀或摆放禽肉案板表面的擦拭标本(31.09％),最低的为粪便标本(13.85％).FluA检出率在12月份最高(28.18％),6、7月份最低(8.59％和8.66％).定西市和陇南市禽流感病毒核酸FluA和H9的检出率显著高于其他市州(x2=4.523 ～ 88.059,P=0.000～0.043 ＜0.05).结论 2015年甘肃省涉禽外环境中存在的禽流感病毒污染主要为H9亚型,未检出H7N9,应加强监测,做好禽流感预警.