纳米孔测序技术在病毒基因组研究中的应用

摘要

Oxford Nanopore Technologies (ONT)公司开发出了商业化的纳米孔测序仪—MinION.相对于二代测序技术平台,MinION的主要特点是读长长、简单快速、便携性高且可以实时分析测序数据.各国的研究人员也已经逐步将MinION应用于病毒检测、病毒全基因组测序、新病毒的发现、病毒准种与进化研究等领域.本文回顾了近几年纳米孔测序技术在病毒基因组研究中的应用,讨论了病毒基因组测序目前存在的问题,并展望了纳米孔测序技术未来的发展前景.%MinION is a commercial nanopore sequencer developed by Oxford Nanopore Technologies.Compared with the second-generation sequencing platform,MinION is characterized by ultra-long reads,simple and fast sequencing,high portability and real-time data analysis.MinION has been widely applied to the fields of virus identification,whole genome sequencing,new virus discovery,quasispecies and virus evolution.In this article,we review the application of nanopore sequencing technology in the research of the viral genome in recent years,and discuss the existing problems.We also present the prospect for the development trend of nanopore sequencing in the future.