第三代测序技术的主要特点及其在病毒基因组研究中的应用

摘要

With the consecutive innovation and application of gene sequencing technology , the third generation sequencing (TGS) based on single molecule real time (SMRT ) sequencing technology has demonstrated a potential in a variety of applications in basic and clinical studies ,including whole genome assembly ,detection of gene structure variation ,epigenetic research ,etc .This review mainly introduces the principle , characteristics and applications of the TGS technology , especially its applications in virus researches .This review also compares the differences between the next generation sequencing (NGS) and TGS ,so as to provide a reference for application based selection for sequencing technology .%随着基因测序技术的创新和应用,新的高通量测序技术不断涌现,以Pacific Biosciences(PacBio)公司的单分子实时测序(single molecule real time sequencing)为代表的第三代测序(third generation sequencing,TGS)技术开始逐渐应用于基因组研究,包括大型基因组拼装、基因结构变异和表观遗传研究等方面.本文主要对T GS技术的原理、特点和应用,特别是在病毒研究中的应用进行介绍,并与第二代测序(next generation sequencing,NGS)技术进行比较,为基因组测序技术的选择及其临床应用提供一定参考.