CYP17A1基因多态性与冠心病遗传易感性的研究

摘要

Objective To discuss the relationship between gene polymorphism of CYP17A1 (rs4409766-locus, rs1004467-locus) and hereditary susceptibility of coronary heart disease (CHD).Methods The patients with CHD or without CHD diagnosed by coronary angiography (CAG) were chosen from the Department of Cardiovasology in Medical College of Qingdao University from June 2014 to Feb. 2016. CYP17A1 (rs4409766-locus, rs1004467-locus) was given analysis of single nucleotide polymorphism (SNP) by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR) in CHD group (n=88) and non-CHD group (n=89). The distributions of genotype frequency and allele frequency were compared between 2 groups.Results The genotype distribution of CYP17A1-rs4409766-locus had no difference between CHD group and non-CHD group (P>0.05), but the difference in genotype distributions of CC and CT+TT had statistical significance between 2 groups (P=0.038). The difference in genotype and allele distributions of CYP17A1-rs1004467-locus had statistical significance between 2 groups (P>0.05).Conclusion The gene polymorphism of CYP17A1-rs4409766-locus is correlated to the incidence of CHD.%目的 探讨CYP17A1(rs4409766,rs1004467)基因多态性与冠状动脉粥样硬化性心脏病(冠心病)遗传易感性之间的关系.方法 选取2014年6月~2016年2月于青岛大学附属医院心血管内科住院并行冠状动脉造影诊断为冠心病和非冠心病的患者为研究对象,应用实时荧光定量PCR(qRT-PCR)技术对冠心病患者88例(CHD组)和非冠心病患者89例(非CHD组),进行CYP17A1基因rs4409766,rs1004467的SNP分析,比较基因型频率及等位基因频率在2组间的分布.结果 CYP17A1基因rs4409766位点的基因型分布在CHD组和非CHD组之间无差别(P>0.05),但是CC与CT+TT基因型分布比较两组间差异有统计学意义(P=0.038);rs1004467位点的基因型和等位基因分布在CHD组和非CHD组之间差异无统计学意义(P>0.05).结论 CYP17A1的rs4409766位点的基因多态性与冠心病的发生有关联.