云南省德宏州2011年HIV-1不同亚型人群分布特征分析

摘要

目的 研究云南省德宏州HIV-1亚型的流行特点.方法 收集2011年1-5月德宏州HIV-1抗体确认阳性血浆样品300份,提取RNA后用巢式PCR对gag基因和env基因的部分片段进行扩增.扩增产物经基因序列测定,所得序列通过构建系统进化树确定HIV-1的分子亚型.结果结合2个基因片段,共有222份样品获得分型结果,主要为C亚型(43 2％,96/222),其他依次为独特重组形式(URF;27.0％,60/222)、CRF01_AE(212％,47/222)、CRF08 BC(5.0％,11/222)、B'(2 3％,5/222)和CRF07 BC( 1.4％,3/222).C亚型是性传播人群和注射吸毒人群中的首要亚型,但不同传播途径人群中亚型分布具有不同的模式.异性传播人群包括该地区检测到6种基因型,主要为C亚型(40 7％,70/172)、CRF 01AE(25.0％,43/172)和多种URF(25.0％,43/172),呈现亚型分布的多样化.注射吸毒人群中包括除B '和CRF07 BC以外的3种亚型及多种URF,主要为C亚型(54.8％,23/42)和URF(38.1％,16/42),呈现亚型相对集中的趋势.URF比例的显著上升成为该地区的另一个流行特点,包括BC重组(41 7％,25/60)和与CRF01 AE相关的重组(58 3％,35/60),这两种URF在性传播和注射吸毒人群中分布的差异无统计学意义.结论 德宏州HIV-1流行株较为复杂,存在5种亚型和多种URF,其中C亚型为主要流行株,不同传播途径亚型分布模式存在差异.%Objective To investigate the distribution of HIV-1 subtypes in Dehong prefecture,Ymnan province,in 2011.Methods 300 HIV-1 positive plasma samples were collected from Jan.2011 to May 2011 in Dehong prefecture.HIV-1 gag genes and env genes were amplified by nestedpolymerase chain reaction (PCR) from viral RNA,After sequencing,the HIV-1 subtypes were determined by phylogenetic analysis.Results Based on the phylogenetic trees of gag gene and env gene fragments,a total of 222 samples were genotyped.Subtype C was the predominant strain in Dehong (43.2％,96/222),followed by unique recombinant forms (URFs,27.0％,60/222),CRF01 _AE ( 21.2％,47/222 ),C R F08_BC ( 5.0％,11/222 ),B,( 2.3％,5/222 ) and CRF07 BC ( 1.4％,3/222).Subtype C strains were predominant in both heterosexually transmitted population and intravenous drug users (IDUs),but different subtype distribution patterns were found in these two populations.All 6 genotypes including subtype C (40.7％,70/172),CRF01_AE (25.0％,43/172),and URFs (25.0％,43/172 ) found in this area among hcterosexually transmitted population,which showed the diversity of genotypes in this population.Except subtype B' and CRF07_BC,the other 3 subtypes and URFs were detected among IDUs,mainly including subtype C (54.8％,23/42) and U R Fs (38.1％,16/42),vhich shoved the concentration trend of genotypes distribution among IDUs.The proportion of URFs increased significantly in this area,including the new BC recombinants (41.7％,25/60) and CRF01_AE relative URFs (58.3％,35/60).However,the distributions of these two URFs among heterosexually transmitted population and IDUs showed no statistical significance.Conclusion The distribution of HIV-1 strains prevailing in Dehong prefecture was diversity,including 5 subtypes and a variety of URFs,of which subtype C was the predominant strain.The distribution patterns of subtype were different among different populations.