聚腺苷二磷酸核糖聚合酶及核因子-κB在重症急性胰腺炎大鼠腺垂体中的表达

摘要

目的 观察聚腺苷二磷酸核糖聚合酶(PARP)及核因子kappa B(NF-κB)在重症急性胰腺炎(SAP)大鼠腺垂体中的表达,并探讨其在SAP大鼠腺垂体损伤中的作用.方法 雄性Wistar大鼠40只,随机(随机数字法)分为5组(n=8):假手术组(SO组)及重症急性胰腺炎组(SAP组)1、3、6、12 h组.胆胰管逆行注射5％牛磺胆酸钠制备重症急性胰腺炎模型.测定腹水量,血清淀粉酶、脂肪酶水平.光镜观察胰腺及垂体病理改变,电镜观察垂体超微结构改变.免疫组化观察垂体PARP及NF-κB的表达.结果 SAP组血清淀粉酶、脂肪酶水平及胰腺病理学评分逐渐增加,均较SO组明显升高(P＜0.05).光镜下可见SAP大鼠腺垂体细胞水肿及坏死；电镜下见SAP大鼠腺垂体出现核固缩、内质网及线粒体肿胀.PARP和NF-κB在SAP大鼠腺垂体中随时间点延长,其表达逐渐增强,均高于SO组表达.结论 在重症急性胰腺炎时,大鼠腺垂体出现病理结构及超微结构损伤,PARP及NF-κB通路的变化可能参与其中.%Objective To investigate the changes of poly-ADP-ribose polymerase (PARP) and NF kappa B (NF-κB) in adenohypophysis in rat model of severe acute pancreatitis (SAP),and their role in the mechanism of adenohypophysis injury in SAP.Methods Forty Wistar rats were randomly (random number) divided into 5 groups:the sham operation group (SO group,n =8),SAP 1 h,3 h,6 h and 12 h groups (n =8 in each group).SAP model was induced by retrograde injection of 5％ sodium taurocholate into the biliopancreatic duct.Serum levels of amylase,lipase and ascites were measured.After sacrifice of experiment rats,pancreas and adenohypophysis tissues were taken for pathological examination under light microscope.Adenohypophysis cells were observed under electronic microscopy as well.PARP and NF-κB expressions in adenohypophysis cell was studied by using immunohistochemisty assay.Results After modelling,serum levels of amylase,lipase and ascites in SAP group increased gradually,which were higher than those in SO group (P ＜ 0.05).Adenohypophysis cell swelling and partial necrosis were observed under light microscope.As the time prolonged,their nuclei became dark and pyknotic more and more,and the endoplasmic reticulum and mitochondrial swelling in adenohypophysis cells were observed under electronic microscopy.The expressions of PARP and NF-κB in SAP group increased gradually,which were higher than those in SO group.Conclusions Significant pathological and ultrastructural injuries were observed in adenohypophysis cells in severe acute pancreatitis.These changes might correlate with PARP and NF-κB signaling pathway.