为明确超级杂交水稻高产形成的分子机理,以超级杂交水稻"Ⅱ优航2号"为试验材料,杂交水稻"汕优63"为对照,运用双向电泳结合质谱技术,比较分析了籽粒灌浆过程中两种不同"源"类型杂交水稻叶片蛋白的差异表达情况.结果显示,两种不同类型水稻叶片蛋白中共有22个蛋白点出现显著差异表达,其中有20个蛋白功能得到鉴定.通过对差异蛋白功能及表达丰度的分析,相对于"汕优63","Ⅱ优航2号"在灌浆期光合代谢、抗逆反应、基因转录表达、细胞生长、能量代谢等生理活动过程中表现出较大优势,是其叶片在籽粒灌浆期"源"优势的主要体现.本研究从差异蛋白水平明确了航天超级杂交稻高产形成的"源"特征,丰富了籽粒灌浆的"源、库、流"理论,为超级杂交水稻育种提供理论依据.%To further clarify molecular metabolism of high yield super-hybrid rice, this paper studied differential expression patterns of leaf proteins in super-hybrid combinations “Ⅱ Youhang No. 2” and hybrid combinations “Shanyou 63” during grain-filling. The proteomic approach, which was based on two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) was used in the study.A total of 22 differentially expressed proteins (at a significant level) were noted, and 20 of which were eventually identified. Further analysis of relative functions and expression abundances showed advantages of “Ⅱ Youhang No. 2” in photosynthetic metabolism,defense, gene-transcript regulation, cell growth, energy metabolism, compared with “Xianyou 63”. The advantages maybe the internal agents of “source” property of “Ⅱ Youhang No. 2” leaf in grain-filling stage. The study revealed the source properties of high yield super-hybrid “Ⅱ Youhang No. 2” at differential proteomic levels. This, to some extent, enriched the source-sink-flow theory and provided a theoretical basis for super-hybrid rice breeding.
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